Goat anti mouse arg 1

Three days after ischemia, animals were perfused with 4% paraformaldehyde in 0.1M phosphate buffer. Brain tissues were postfixed for 4–6h and immersed in gradient sucrose solutions for 24–48h at 4°C until they sank. Sections were cut (20μm thickness) with a cryostat and prepared for imunostaining. The slides were blocked with 1% bovine serum albumin containing 0.3% Triton X-100 for 1h. Sections were then incubated (overnight at 4°C) with the following primary antibodies: rabbit anti mouse Iba1 (1:500; Wako), goat anti mouse Arg1 (1:100; Santa Cruz), rabbit anti mouse iNOS (1:200; Abcam), mouse anti-8 hydroxyguanosine (8-OHG, Abcam, 1:200), mouse anti-NeuN (1:500, Chemicon), rabbit anti-Caspase 3(1:200, Abcam). The sections were then incubated (for 1h at room temperature) with the respective secondary fluorescent antibodies (all 1:500; Jackson ImmunoResearch) to visualize the primary antibodies. Images were acquired with a confocal microscope (Olympus).