Cells were seeded at 60% confluency in 10% FBS‐containing DMEM for 24 h in p60 plates. AURKA and eIF4E were transiently silenced by using siAURKA and sieIF4E (Invitrogen) for a total of 48 h. A negative siRNA control (Ambion, Austin, TX, USA) was used in each experiment. Transfection of cells was achieved by using a LipoJet reagent (SignaGen) according to the manufacturer's instructions. Following 24‐h transfection, medium was replaced with DMEM, supplemented with 5% FBS and antibiotics for another 24 h prior to harvesting. Validation of AURKA and eIF4E knockdown was assessed by qPCR and western blot analyses.
Siaurka
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SiAURKA is a specialized piece of laboratory equipment designed for targeted applications. It is a compact and versatile instrument that serves a core function within its intended use. The details of its specific capabilities and applications are limited in this factual presentation.
Automatically generated - may contain errors
2 protocols using siaurka
1
Transient Silencing of AURKA and eIF4E
2
Silencing AURKA and eIF4E in cells
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