Monoclonal antibody (Mab) BU14, which recognizes the C-terminal region of HHV-6 U14, was described previously [9 (link)]. Mab AgQ1–119, for HHV-6A gQ1 was described previously [36 (link)] Anti-HA antibody (clone HA-7; Sigma), rabbit polyclonal antibody against EDD (BETHYLor Abcam), and anti–α-tubulin antibody (Sigma) were purchased from the indicated suppliers. Alexa Fluor 488- or 594-conjugated F(ab’)2 fragment of donkey anti-rabbit or-mouse immunoglobulin G (IgG) (Invitrogen) was used as a secondary antibody.
Anti ha antibody clone ha 7
Manufactured by Merck Group
The Anti-HA antibody (clone HA-7) is a laboratory reagent used for the detection and identification of proteins tagged with the Hemagglutinin (HA) epitope. It is a monoclonal antibody that specifically binds to the HA tag, which is a widely used protein tag in molecular biology experiments.
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Lab products found in correlation
2 protocols using anti ha antibody clone ha 7
1
Monoclonal Antibodies for HHV-6 Research
2
Endogenous Tagging of Hypothetical Proteins
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The 30 hypothetical proteins whose transcripts were highly enriched in S phase (Supplementary Table S1 ), the 21 orphan kinesins and kinetoplastid-specific kinesins (Supplementary Table S2 ), and the T. brucei Fidgetin homolog (Tb927.5.1870) were each endogenously tagged with a triple HA epitope by the PCR-based gene tagging method (28 (link)) in the 427 cell line. Transfectants were selected with 1 μg/ml puromycin and cloned by limiting dilution in a 96-well plate containing SDM-79 medium supplemented with 20% heat-inactivated fetal bovine serum. Western blotting was carried out with anti-HA antibody (clone# HA-7, Catalog # H3663, Sigma-Aldrich) to confirm the tagging of proteins. Cells were then immunostained with FITC-conjugated anti-HA antibody (1:400 dilution, Clone# HA-7, Catalog# H7411, Sigma-Aldrich) and imaged under an inverted fluorescence microscope.
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