Secondary antibodies against rabbit 7074s and mouse 7076s igg

Manufactured by Cell Signaling Technology

Secondary antibodies against rabbit (7074S) and mouse (7076S) IgG. These antibodies are used to detect and amplify the signal from primary antibodies that bind to rabbit or mouse immunoglobulin G (IgG) in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Protein g coupled dynabeads, by Thermo Fisher Scientific (2 mentions) Thiolutin, by Abcam (2 mentions) β actin gtx109639, by GeneTex (2 mentions) 5 foa, by Cayman Chemical (2 mentions) Iaid tag m214 3, by MBL Life Science (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Drop out mix for yeast synthetic medium, by US Biological (1 mentions)

2 protocols using secondary antibodies against rabbit 7074s and mouse 7076s igg

Genotypes and Reagents for Yeast Study

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Genotypes of strains and yeast plasmids used in this work are listed in Table S3 and S4. All chemicals used in this study were purchased analytical grade from either Sigma-Aldrich or Carl Roth except for the following: Drop Out Mix for yeast synthetic medium (SD) was from US Biological Life Sciences (D9543-01); 5-FOA was bought from Cayman Chemical (17318); Ampure XP beads were from Beckman Coulter (A63881); Protein-G coupled dynabeads from Thermo Fisher Scientific (10009D) and Thiolutin from Abcam (ab143556). Secondary antibodies against rabbit (7074S) and mouse (7076S) IgG coupled to horseradish peroxidase (HRP) were purchased from Cell Signaling. Antibodies against H3K56ac were from Active Motif (39281), G6PDH (A9521) and Myc (MABE282) from Sigma, iAID-tag (M214-3) from MBL International and β-actin (GTX109639) from GeneTex. Antibody dilutions were as suggested by the manufacturer (Table S12).

Kochan D.Z., Mawer J.S., Massen J., Tishinov K., Parekh S., Graef M., Spang A, & Tessarz P. (2021). The RNA-binding protein Puf5 contributes to buffering of mRNA upon chromatin-mediated changes in nascent transcription. Journal of Cell Science, 134(15), jcs259051.

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Yeast Genetic Manipulation Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genotypes of strains and yeast plasmids used in this work are listed in Supplementary Table 3 and4. All chemicals used in this study were purchased analytical grade from either Sigma Aldrich or Carl Roth except for the following: Drop Out Mix for yeast synthetic medium was from US Biological Life Sciences (D9543-01). 5-FOA was bought from Cayman Chemical (17318). Ampure XP beads were from Beckman Coulter (A63881), Protein-G coupled dynabeads from Thermo Fisher (10009D) and Thiolutin from Abcam (ab143556). Secondary antibodies against rabbit (7074S) and mouse (7076S) IgG coupled to HRP were purchased from Cell Signaling. Antibodies against H3K56ac were from Active Motif (39281), G6PDH (A9521) and c-Myc (MABE282) from Sigma, iAID-tag (M214-3) from MBL International and βactin (GTX109639) from GeneTex.

Kochan D.Z., Mawer J.S.P., Tishinov K., Parekh S., Massen J., Graef M., Spang A., & Tessarz P. (2020). The RNA-binding protein Puf5 buffers mRNA levels against chromatin-mediated changes in nascent transcription.

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