Flag m2 antibody f1804

Manufactured by Merck Group

The FLAG M2 antibody F1804 is a monoclonal antibody that recognizes the FLAG epitope, a small peptide tag that can be fused to recombinant proteins for detection and purification purposes. The antibody is designed for use in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunoaffinity chromatography, to detect and purify FLAG-tagged proteins.

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Lab products found in correlation

Ab51013, by Abcam (1 mentions) Ab184565, by Abcam (1 mentions) A2103, by Merck Group (1 mentions) Gtx118991, by GeneTex (1 mentions) Mg132 hy 13259, by MedChemExpress (1 mentions) Ha 3724, by Cell Signaling Technology (1 mentions) Abs137975, by Absin (1 mentions)

2 protocols using flag m2 antibody f1804

Influenza Virus Protein Interactions

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Polymerase reconstitution assays, vPol interaction assays (co-immunoprecipitations), and western blot analyses were all performed using standard described methods^{15 (link)}. FLAG-tagged constructs were detected using FLAG M2 antibody F1804 (Sigma; 1:2000 dilution), PB2 was detected using a custom rabbit polyclonal anti-serum (1:2000 dilution), PA was detected using antibody GTX118991 (Genetex; 1:2000 dilution), actin was detected using antibody A2103 (Sigma; 1:3000 dilution), and ANP32A and ANP32B were detected using antibodies ab51013 and ab184565 (Abcam; 1:1000 dilution), respectively.

Domingues P., Eletto D., Magnus C., Turkington H.L., Schmutz S., Zagordi O., Lenk M., Beer M., Stertz S, & Hale B.G. (2019). Profiling host ANP32A splicing landscapes to predict influenza A virus polymerase adaptation. Nature Communications, 10, 3396.

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Antibody Validation for Protein Analysis

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Primary antibodies against Myc (sc-40), β-catenin (sc-7199), VBP1 (sc-390465), and pVHL (sc-135657) were purchased from Santa Cruz Biotechnology. Antibodies against TCF7L2 (2569), GFP (2555), and HA (3724) were obtained from Cell Signaling Technology. The antibody against TCF7L1/L2 (ab12065) was provided by Abcam. The antibody against GAPDH (D110016) was purchased from BBI Life Sciences (Crumlin, UK). The antibody against histone H3 (P30266) was obtained from Abmart. The FLAG M2 antibody (F1804) was provided by Sigma-Aldrich. The antibody against β-actin (abs137975) was obtained from Absin Bioscience. Small molecules CHX (66-81-9) and BIO (667463-62-9) were purchased from Sigma-Aldrich. MG132 (HY-13259) was from MedChemExpress.

Zhang H., Rong X., Wang C., Liu Y., Lu L., Li Y., Zhao C, & Zhou J. (2021). VBP1 modulates Wnt/β-catenin signaling by mediating the stability of the transcription factors TCF/LEFs. The Journal of Biological Chemistry, 295(49), 16826-16839.

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