Toluidine blue dye

Rifampicin, compound 48/80, disodium cromoglycate (cromolyn), p-nitrophenyl-N-acetyl-β-D-glucosaminide [PN-(GlcNAc)₂], thiazolyl blue tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), toluidine blue dye, CaCl₂, MgCl₂, NaHCO₃, and glucose were purchased from Sigma-Aldrich Korea (Yongin, Korea). The histamine enzyme-linked immunosorbent assay (ELISA) kit was obtained from IBL International GmbH (Hamburg, Germany). We purchased the human PGD₂ ELISA kit from Cusabio (Wuhan, China) and the TNF-α ELISA kit from eBioscience (San Diego, USA). The total RNA isolation kit was purchased from GeneAll (Seoul, Korea). Fluo-3 AM was obtained from Molecular Probes (Eugene, OR, USA).

Human keratinocyte HaCaT cells were obtained from the Cell Lines Service (Eppelheim, Germany). Human primary neonatal keratinocyte HEKn cells were purchased from ThermoFisher Scientific Korea (Seoul, Korea). HaCaT cells were maintained in DMEM medium (1.8 mM CaCl₂) supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) and HEKn cells were cultured in EpiLife medium supplemented with Human Keratinocyte Growth Supplement (HKGS) (ThermoFisher Scientific Korea). Agerarin was isolated as described previously [15 (link)]. 2,4-Dinitrochlorobenzene (DNCB) was obtained from Sigma–Aldrich (St. Louis, MO, USA). TNFα and IFNγ were obtained from Prospec-Tany Technogene Ltd. (Ness-Ziona, Israel). The hematoxylin and eosin (H&E) stain kit and toluidine blue dye were obtained from Sigma–Aldrich. The Firefly Luciferase Assay System was from Promega (Madison, WI, USA). Antibodies against phospho-JAK1 (Y1034/1035), JAK1, phospho-JAK2 (Y1007/1008), JAK2, phospho-STAT3 (Y705), and STAT3 were purchased from Cell Signaling Technology (Beverly, MA, USA).Antibodies against FLG and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-FLG antibody for immunofluorescent staining was from BioLegend (San Diego, CA, USA).