All procedures were performed at 45°C on a microscope stage heated with a thermoplate (TP-110R-100; Tokai Hit, Japan). The cell culture was poured into a tunnel chamber assembled by taping a coverslip (Nakane and Nishizaka, 2017 (link)), and both ends of the chamber were sealed with nail polish to keep from drying the sample. The position of the cell was visualized by infrared light from a halogen lamp with a bandpass filter (FBH850/40; Thorlabs) at a fluence rate of 1 μmol m−2 s−1. The cells were subjected to lateral light stimulus by an LED from the right side of the microscope stage at an angle of 5°. White LEDs at 20 and 500 μmol m−2 s−1 were used as moderate and strong light stimuli for phototaxis, respectively. Blue, teal, green, orange, red, and far-red light were applied by a monochromatic LED, M450LP1, M490L4, M530L3, M625L3, and M730L4 (Thorlabs), respectively. The LED light was collimated by the condenser lens and combined by dichroic mirrors (FF470-Di01, FF509-FDi01, FF560-FDi01, FF685-Di02; Semrock) to apply multicoloured light simultaneously. The wavelength of the resultant light was measured by a spectrometer (BIM-6002A, BroLight, China). Light intensity was measured with a power metre (Q82017A; Advantest, Japan).
Ff509 fdi01
Manufactured by IDEX Corporation
The FF509-FDi01 is a laboratory equipment product designed for precision fluid dispensing. It features a compact and durable design for reliable operation in various laboratory settings. The core function of this product is to accurately and consistently deliver measured volumes of liquids or other fluid samples.
Automatically generated - may contain errors
Lab products found in correlation
M450lp1, by Thorlabs (2 mentions)
Fbh850 40, by Thorlabs (2 mentions)
Ff470 di01, by IDEX Corporation (2 mentions)
Ff560 fdi01, by IDEX Corporation (2 mentions)
Tp 110r 100, by Tokai Hit (2 mentions)
Ff685 di02, by IDEX Corporation (2 mentions)
Axiovert 135, by Zeiss (1 mentions)
Blp01 532r, by IDEX Corporation (1 mentions)
Plan apo vc 100, by Nikon (1 mentions)
3 protocols using ff509 fdi01
1
Microscopic Phototaxis Assay at Optimal Temperature
2
Single-Molecule Fluorescence Microscopy Setup
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An optical microscope (Zeiss Axiovert 135) is used to image fluorescent samples of QDs. A two-axis piezo stage (P-542.2SL, Physik Instrumente) is used to position the sample. For illumination, a 473 nm pulsed picosecond laser diode (Edinburgh Instruments) is used, coupled to a single-mode fibre. The repetition rate of this laser is set to 20 MHz. A 1.4 numerical aperture objective lens (Plan Apo Vc 100 × , Nikon) is used to tightly focus the illuminating laser. The fluorescence is collected by the same objective lens and filtered by dichroic mirrors and filters (FF509-FDi01, SP01-785RS, BLP01-532R, Semrock). A Galilean beam expander (BE05-10-A, Thorlabs) is placed following the relay lens to magnify the imaged fluorescence spot on to a fibre bundle (A.R.T. Photonics GmbH, Germany). This fibre bundle consists of multimode 100/110 μm core/clad fibres, fused on one side and fan-out to individual multimode fibres on the other side, and is used to guide photon from an imaged spot to 15 fibre coupled single-photon avalanche photodiodes (SPCM-AQ4C, Perkin-Elemer). For a detailed characterization of the fibre bundle setup see Supplementary Note 4 . The overall detection efficiency of our setup is 12%, and further details about the efficiency are found in the Supplementary Note 1 and Supplementary Table 1 .
3
Phototaxis Analysis of Motile Cells
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All procedures were performed at 45 °C on a microscope stage heated with a thermoplate (TP-110R-100; Tokai Hit, Japan). The cell culture was poured into a tunnel chamber assembled by taping a coverslip (7) (link), and both ends of the chamber were sealed with nail polish to keep from drying the sample. The position of the cell was visualized by infrared light from a halogen lamp with a bandpass filter (FBH850/40; Thorlabs) at a fluence rate of 1 μmol m -2 s -1 . The cells were subjected to lateral light stimulus by an LED from the right side of the microscope stage at an angle of 5 degrees. White LEDs at 20 and 500 μmol m -2 s -1 were used as moderate and strong light stimuli for phototaxis, respectively. Blue, teal, green, orange, red, and far-red light were applied by a monochromatic LED, M450LP1, M490L4, M530L3, M625L3, and M730L4 (Thorlabs), respectively. The LED light was collimated by the condenser lens and combined by dichroic mirrors (FF470-Di01, FF509-FDi01, FF560-FDi01, FF685-Di02; Semrock) to apply multicoloured light simultaneously. The wavelength of the resultant light was measured by a spectrometer (BIM-6002A, BroLight, China). Light intensity was measured with a power metre (Q82017A; Advantest, Japan).
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