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CRL-1992 is a cell line derived from human embryonic kidney cells. It is a commonly used laboratory tool for a variety of research applications.

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Lab products found in correlation

Elispot plate reader, by Autoimmun Diagnostika (1 mentions)

2 protocols using crl 1992

1

Cell Culture Protocols for Leukemia and Colon Cancer

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The HLA-A*02:01 positive leukemia cell line T2 (ATCC® CRL-1992™) was kindly provided by Professor Y. van Kooyk (VU University Medical Center, Amsterdam, The Netherlands). Cells were kept in culture using RPMI-1640 medium supplemented with 10% FBS, 2 mM GlutaMAX and 100 μg/mL penicillin/streptomycin. The colon cancer carcinoma cancer cell line SW948 (ATCC® CCL-237™) was kindly provided by Professor Fabio Dall’Olio Department of Experimental Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy). Cells were cultured in Leibovitz’s L-15 medium supplemented with 10% FBS.
Silva Z., Ferro T., Almeida D., Soares H., Ferreira J.A., Deschepper F.M., Hensbergen P.J., Pirro M., van Vliet S.J., Springer S, & Videira P.A. (2020). MHC Class I Stability is Modulated by Cell Surface Sialylation in Human Dendritic Cells. Pharmaceutics, 12(3), 249.
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2

Measuring IFNg Secretion by ELISpot

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IFNg secretion was measured by enzyme-linked immunospot assay (ELISpot; R&D Systems). T cells previously expanded against neoantigen peptides were loaded onto ELISpot plates at 25,000 cells per well and stimulated with a T2 cell line (CRL-1992; ATCC) pulsed with 10 mg/mL peptide at a ratio of 4:1 T2-to-T cells. IFNg spots were detected after 24 hours of stimulation and counted with an automated ELISpot Plate Reader (Autoimmun Diagnostika).
Lazdun Y., Si H., Creasy T., Ranade K., Higgs B.W., Streicher K, & Durham N.M. (2021). A New Pipeline to Predict and Confirm Tumor Neoantigens Predict Better Response to Immune Checkpoint Blockade. Molecular cancer research : MCR, 19(3).
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