Scnn1a tg3 cre

Manufactured by Jackson ImmunoResearch

The Scnn1a-Tg3-Cre is a transgenic mouse line that expresses Cre recombinase under the control of the sodium channel, nonvoltage-gated 1 alpha (Scnn1a) gene promoter. The core function of this product is to provide a tool for cell-type-specific gene manipulation in the mouse model.

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Lab products found in correlation

Vip ires cre, by Jackson ImmunoResearch (2 mentions) Gad2 ires cre, by Jackson ImmunoResearch (1 mentions) Jax stock, by Jackson ImmunoResearch (1 mentions) Pv ires cre, by Jackson ImmunoResearch (1 mentions) Sst ires cre, by Jackson ImmunoResearch (1 mentions) Ai34d, by Jackson ImmunoResearch (1 mentions) Pv cre, by Jackson ImmunoResearch (1 mentions) Som ires cre, by Jackson ImmunoResearch (1 mentions)

4 protocols using scnn1a tg3 cre

Tracing Cell-Type Specific Neural Circuits

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All animal procedures complied with the animal care standards set forth by the US National Institutes of Health and have been approved by the Institutional Animal Care and Use Committee at the Institute of Neuroscience, Chinese Academy of Sciences. Mice were raised in a specific pathogen-free environment, group housed under a 12-12 h light-dark cycle, and with food and water provided at libitum from the cage lid. Health status was routinely checked and only naïve mice (no previous procedures or tests) were used. Wild-type ICR mice were used for IUE experiments; otherwise mice on C57/BL6 background were used. The day of vaginal plug was designated as embryonic day 0 (E0), while the day of birth was designated as postnatal day 0 (P0). Scnn1a-Tg3-Cre (full name: B6;C3-Tg (Scnn1a-cre)3Aibs/J; RRID:IMSR_JAX:009613) and Ai34D (full name B6;129S-Gt (ROSA)26Sor tm34.1(CAG-Syp/tdTomato)Hze /J; RRID:IMSR_JAX:012570) mice were obtained from the Jackson Laboratory, and crossed to generate Scnn1a-Tg3-Cre +/-::Ai34D +/+ mice. Both male and female P14 mice were used for all experiments. The numbers of mice used are as indicated in figure legends; 3 or more mice were used per experimental condition.

Wang M., Yu Z., Li G, & Yu X. (2020). Multiple Morphological Factors Underlie Experience-Dependent Cross-Modal Plasticity in the Developing Sensory Cortices. Cerebral cortex (New York, N.Y. : 1991), 30(4).

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Genetically Engineered Mice for Neuronal Labeling

Cited 2 times

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We obtained Gad2-IRES-Cre (Taniguchi et al., 2011 (link), Jax stock #010802), Scnn1a-tg3-cre (Madisen et al., 2010 (link), Jax stock #009613), and Ai14 mice (Madisen et al., 2010 (link), Jax stock #007914) from the Jackson Laboratory. These mice were crossed to generate Gad2-Ai14 and Scnn1a-Ai14 mice. Both sexes were used. In Gad2-Ai14 mice, most cortical inhibitory neurons express tdTomato (Taniguchi et al., 2011 (link)). In Scnn1a-Ai14 mice, layer 4 neurons express tdTomato (Madisen et al., 2010 (link)).

Hayashi A., Yoshida T, & Ohki K. (2018). Cell Type Specific Representation of Vibro-tactile Stimuli in the Mouse Primary Somatosensory Cortex. Frontiers in Neural Circuits, 12, 109.

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Characterizing Transgenic Mouse Lines

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All experiments were approved by and in accordance with Harvard Medical School IACUC protocol number IS00001269. C57Bl/6 mice were used for breeding with transgenic mice. Transgenic mice, NDNF-dgCre (Jax stock number: 028536), Vipr2-IRES-Cre-D (stock number: 031332), Scnn1a-Tg3-Cre (Jax stock number: 009613), VIP-IRES-Cre (Jax stock #010908), PV-IRES-Cre (Jax stock #017320), SST-IRES-Cre (Jax stock # #013044), Ai14 (expressing tdTomato, stock number: 007909), Sun1-GFP (Jax stock #021039) are available at Jackson Laboratories. Animals were group housed and maintained under standard, temperature-controlled laboratory conditions. Mice were kept on a 12:12 light/dark cycle and received water and food ad libitum. For experiments during development, mice were injected at ~P1 and experiments conducted between ages P13-P15. In the case of adults, mice were injected at P30, and experiments conducted between ages P45-P50. Both female and male were used in the entire study and similar results were obtained in both sexes.

Ibrahim L.A., Huang S., Fernandez-Otero M., Sherer M., Qiu Y., Vemuri S., Xu Q., Machold R., Rudy B, & Fishell G. (2021). Bottom-up inputs are required for the establishment of top-down connectivity onto cortical layer 1 neurogliaform cells. Neuron, 109(21), 3473-3485.e5.

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Genetic Mouse Lines for Neuroscience Research

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All procedures were carried out in accordance with the National Institutes of Health (NIH) Guidelines for the Care and Use of Laboratory Animals and approved by the Michigan State University Institutional Animal Care and Use Committee (IACUC). We used the following mouse lines in this study: Crl: CD1 (ICR) (Charles River: 022), Ai14 (Jackson Labs: 007908) (Madisen et al. 2010) , PV-Cre (Jackson Labs: 008069) (Hippenmeyer et al. 2005) , SOM-IRES-Cre (Jackson Labs: 013044) (Taniguchi et al. 2011) , VIP-IRES-Cre (Jackson Labs: 010908) (Taniguchi et al. 2011 ), Scnn1a-Tg3-Cre (Jackson Labs: 009613) (Madisen et al. 2010) , Syt7 knockout (Jackson Labs: 004950) (Chakrabarti et al. 2003) , Ntsr1-Cre (MMRRC: 017266-UCD) (Gong et al. 2007 ), Rbp4-Cre (MMRRC: 031125-UCD) (Gong et al. 2007) , and 5HT3a-EGFP (MMRRC: 000273-UNC). The Ntsr1-Cre, Rbp4-Cre, Scnn1a-Tg3-Cre, Syt7 knockout, and 5HT3a-EGFP mouse lines had ICR genetic backgrounds. All mice, except for ICR, Syt7 knockout, and 5HT3a-EGFP mice, were bred by crossing homozygous or heterozygous Cre mice with homozygous Ai14 reporter mice, resulting in experimental mice that were heterozygous for the indicated genes. Animals were group-housed with same-sex littermates in a dedicated animal care facility maintained on a 12:12 hour light-dark cycle. Food and water were available ad libitum. We used both male and female mice in this study.

Martinetti L.E., Bonekamp K.E., Autio D.M., Kim H., & Crandall S.R. (2021). Short-term facilitation of long-range corticocortical synapses revealed by selective optical stimulation.

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