Neocuproin

Manufactured by Merck Group

Sourced in Germany

Neocuproin is a chemical compound that functions as a chelating agent. It is used in analytical chemistry and laboratory settings to detect and quantify the presence of copper ions in various samples.

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6 protocols using neocuproin

Comprehensive Chemical Analyses Protocol

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Water, m ethanol, acetonitrile (HPLC grade), Folin-Ciocalteu reagent, ethanol, and formic acid were obtained from Merck (Darmstadt, Germany). Phosphoric acid, TROLOX (6-hydroxy-2,5,7,8 acid tetramethylchroman-2-carboxylic acid), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), neocuproin (≥98%), quercetin (>95%), copper (II and III) chloride (grade pa), potassium persulfate, chlorogenic acid (≥95%), iron (III) chloride, calcium chloride, ammonium acetate, and m ethanol were obtained from Sigma-Aldrich (Steinheim, Germany). Sodium carbonate and TPTZ (2,4,6-tripyridyl-1,3,5-triazine) were obtained from Supelco (Darmstadt, Germany).

Valdebenito A., Nahuelcura J., Santander C., Cornejo P., Contreras B., Gómez-Alonso S, & Ruiz A. (2022). Physiological and Metabolic Effects of the Inoculation of Arbuscular Mycorrhizal Fungi in Solanum tuberosum Crops under Water Stress. Plants, 11(19), 2539.

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Antioxidant-rich Lipid-Hyaluronate Formulation

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Lipoid S75 (consisting of ~70% of soy phosphatidylcholine, 9% phosphatidylethanolamine and 3% lyso-phosphatidylcholine) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Sodium hyaluronate with low molecular weight (200–400 kDa) and a polydispersity of 1.4 Mw/Mn, was purchased from DSM Nutritional Products AG Branch Pentapharm (Switzerland). Tween 80, glycerol, DPPH radical (2,2-diphenyl-1-picrylhydrazyl), ferric chloride hexahydrate, gallic, chlorogenic, and ascorbic acids, catechin, tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), quercetin, neocuproin, Folin–Ciocalteu’s phenol reagent, 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) and pyrocatechol violet were purchased from Sigma-Aldrich (Milan, Italy). Potassium hexacyanoferrate (III) was obtained from Merck (Darmstadt, Germany). Anhydrous sodium acetate, copper sulfate pentahydrate and copper chloride dihydrate were obtained from VWR Chemicals BDH^® (Frankfurt, Germany). Cyanidin 3-O-glucoside (≥95%) and delphinidin 3-O-glucoside (≥95%) were purchased from Extrasynthese (Lyon, France). Glacial acetic acid and phosphoric acid (85%) were acquired from J.T. Baker (Mallinckrodt Baker Inc., Utrecht, The Netherlands). All reagents and plastics for cell culture were purchased from Life Technologies Europe (Monza, Italy).

Castangia I., Manca M.L., Allaw M., Hellström J., Granato D, & Manconi M. (2021). Jabuticaba (Myrciaria jaboticaba) Peel as a Sustainable Source of Anthocyanins and Ellagitannins Delivered by Phospholipid Vesicles for Alleviating Oxidative Stress in Human Keratinocytes. Molecules, 26(21), 6697.

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Antioxidant Capacity Evaluation of Petunidin-3-glucoside

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Standard petunidin-3-glucoside (98.95%) was obtained from PhytoLab (Vestenbergsgreuth, Germany). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) (97%), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) (>98%), gallic acid (97.5–102.5% titration), chlorogenic acid (≥95%), neocuproin (≥98%), DPPH (2,2-diphenyl-1-picrylhydrazyl), Folin–Ciocalteu reagent, AAPH (2,2′-azobis-2-methyl-propanimidamine, dihydrochloride) (97%), fluorescein (95%), monobasic potassium phosphate (KH₂PO₄), dibasic potassium phosphate (K₂HPO₄), potassium persulfate (K₂O₈S₂), ammonium acetate and copper (II) chloride (CuCl₂) were obtained from Sigma-Aldrich (Steinheim, Germany). Water (HPLC grade), ethanol (HPLC grade), methanol (HPLC grade), acetonitrile (HPLC grade), formic acid (p.a. grade) and sodium carbonate (K₂O₈S₂) were obtained from Merck (Darmstadt, Germany).

Bravo C., Peña F., Nahuelcura J., Vidal C., González F., Jiménez-Aspee F., Bustamante L., Contreras B, & Ruiz A. (2023). Stability of Phenolic Compounds, Antioxidant Activity and Color Parameters in Colored-Flesh Potato Chips. Molecules, 28(16), 6047.

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Enzymatic Assay for Protease Activity

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Esperase (Novozyme), trypsin, Nα-benzoyl-L-arginine 4-nitroanilide hydrochloride (BAPNA), casein, Folin-Ciocalteu´s phenol reagent, murexide, trichloroacetic acid (TCA), neocuproin, CuCl2, CuSO4, Na2CO3, NH₄CH₃CO₂ were purchased from Sigma Chemical Co. (St. Louis, Mo., U.S.A).

Kocazorbaz E.K., KANATSIZOĞLU M., Çeker T., & Menfaatli E. (2020). Enzymatic Hydrolysis of Stingray (Dasyatis pastinaca) Muscle and Evaluation of Antioxidant and Copper Chelating Activities of the Hydrolysates. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, 7(2), 680-687.

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Enzyme Inhibition and Antioxidant Assays

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The following reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA): AlCl₃, amberlite XAD7 HP, α-amylase from porcine pancreas (A3176; EC 3.2.1.1), α-glucosidase from Saccharomyces cerevisiae (G5003; EC 3.2.1.20), DPPH (2,2-diphenyl-1-picrylhydrazyl radical), DMAC (4-[dimethylamino]cinnamaldehyde), 3,5-dinitrosalicylic acid, catechin, caffeic acid, CuCl₂, hypoxanthine, indomethacin, linoleic acid (L-5900), lipase from porcine pancreas type II (L-3126; EC 3.1.1.3), Na₂CO₃, nimesulide, 4-nitrophenyl-α-d-glucopyranoside, nitro blue tetrazolium salt, p-nitrophenyl palmitate, sodium acetate, soy lipooxygenase-15 (L7395; EC 1.13.11.12), starch, 2,4,6-tri(2-pyridyl)1,3,5-triazine (TPTZ), triton X-100, tris, and ursolic acid. From Merck (Darmstadt, Germany): Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), FeCl₃∙6H₂O, neocuproin, potassium sodium tartrate and HPLC-grade methanol. Ammonium acetate was from JT Baker (Xalostoc, Mexico). The sodium and potassium phosphate salts were from Scharlau Chemicals (Barcelona, Spain). Orlistat was from Laboratorio Chile (Santiago, Chile). Culture medium, antibiotics and fetal bovine serum were obtained from Invitrogen Corp. (Waltham, MA, USA). Ultrapure water was obtained using a Barnsted EasyPure water filter (Thermo Scientific, Marietta, OH, USA).

Jiménez-Aspee F., Theoduloz C., Soriano M.D., Ugalde-Arbizu M., Alberto M.R., Zampini I.C., Isla M.I., Simirgiotis M.J, & Schmeda-Hirschmann G. (2017). The Native Fruit Geoffroea decorticans from Arid Northern Chile: Phenolic Composition, Antioxidant Activities and In Vitro Inhibition of Pro-Inflammatory and Metabolic Syndrome-Associated Enzymes. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(9), 1565.

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Evaluation of Antioxidant and Enzyme Inhibitory Activities

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From Sigma-Aldrich (St. Louis, MO, USA): Amberlite XAD7^®, α-amylase from porcine pancreas (A3176; EC 3.2.1.1), α-glucosidase from Saccharomyces cerevisiae (G5003; EC 3.2.1.20), DPPH (2,2-diphenyl-1-picrylhydrazyl radical), 3,5-dinitrosalicylic acid, catechin, CuCl₂, lipase from porcine pancreas type II (L-3126; EC 3.1.1.3), 4-nitrophenyl-α-D-glucopyranoside, p-nitrophenyl palmitate, quercetin, sodium acetate, starch, TPTZ (2,4,6-tri(2-pyridyl)1,3,5-triazine), and triton X-100. From Merck (Darmstadt, Germany): ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), FeCl₃ × 6H₂O, neocuproin, potassium sodium tartrate, and HPLC-grade methanol. Ammonium acetate was from JT Baker (Xalostoc, Mexico). The following standards were from PhytoLab (Vestenbergsgreuth, Germany): malvidin-3-glucoside chloride (89728, 99.3% purity), quercetin-3-glucoside (89230, 99.1% purity), and chlorogenic acid (89175, 98.9% purity). Orlistat was from Laboratorio Chile (Santiago, Chile).

Chamorro M.F., Reiner G., Theoduloz C., Ladio A., Schmeda-Hirschmann G., Gómez-Alonso S, & Jiménez-Aspee F. (2019). Polyphenol Composition and (Bio)Activity of Berberis Species and Wild Strawberry from the Argentinean Patagonia. Molecules, 24(18), 3331.

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