Anti f4 80

Liver tissue specimens were homogenized in RIPA buffer (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) and the total protein concentration of each sample was measured by a BCA protein assay kit (Thermo Fisher Scientific). Protein lysate was mixed with Laemmli sample loading buffer (BioRad, CA, USA), and equal amounts of protein were separated by SDS-PAGE. The isolated proteins were transferred to PVDF membranes (BioRad), which were blocked using Blocking One (Nacalai Tesque, Kyoto, Japan). Antibodies were obtained as follows: anti-NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1) from Abcam (ab2346, Cambridge, UK), anti-lipopolysaccharide binding protein (LBP) from Proteintech (23559-1-AP, Tokyo, Japana), anti-F4/80 from Hycult Biotech (HM1066, Uden, Yhe Netherlamds,), anti-hexanoyl-lysine (HEL) from JaiCA, (MHL-021P, Shizuoka, Japnan), and anti-β-actin from Sigma (A5441, Deisenhofen, Germany). Target proteins were visualized using Chemi-Lumi One Super (Nacalai Tesque), and were quantified using ImageJ software (NIH, MD, USA).