Mouse anti her 2

For cell stimulation, anti‐CD3ε (clone 145‐2C11) and anti‐CD28 (clone 37.51) antibodies were purchased from BD Biosciences. Antibodies against p‐(Y701) STAT1 (clone 58D6), p‐(Y694) STAT5 (D47E7) XP^® and STAT1 were from Cell Signaling. Anti‐actin (clone ACTN05) was purchased from Thermo Fisher Scientific. The mouse antibody against PTPN2 (clone 6F3) was provided by M. Tremblay (McGill University). For immunofluorescence staining and immunohistochemistry, rabbit anti‐CD3ε and mouse anti‐HER‐2 from Abcam were used. Recombinant human IL‐2, murine IL‐7 and IL‐15 used for T‐cell stimulation or IFNγ used for stimulating tumour cells were purchased from the NIH or PeproTech, respectively. RetroNectin was purchased from Takara, Dnase I and doxycycline from Sigma‐Aldrich. The mouse anti‐nuclear antibodies Ig's (total IgA+G+M) ELISA Kit (Alpha Diagnostic Int.) and the Transaminase II Kit (Wako Pure Chemicals) were used according to the manufacturer's instructions. FBS was purchased from Thermo Scientific; Dulbecco‐Phosphate‐Buffered Saline (D‐PBS), RPMI 1640, DMEM, MEM non‐essential amino acids and sodium‐pyruvate were from Invitrogen, and collagenase type IV was purchased from Worthington Biochemical.

Cells were lysed with RIPA buffer and centrifuged at 13,800 × g for 10 min. Protein lysates were separated by SDS/PAGE, blotted onto a PVDF membrane, and probed overnight at 4 °C with antibodies against rabbit anti-SHCBP1 (1:1000; Sigma), mouse anti-HER2 (1:1000, Abcam), mouse anti-Flag (1:1000, Sigma), mouse anti-HA (1:1000, Invitrogen), rabbit anti-MISP (1:1000, Abcam), rabbit anti-PLK1 (1:1000, Proteintech), rabbit anti-Shc (1:1000, Abcam), mouse anti-β-actin (1:1000, Proteintech), mouse anti-GAPDH (1:1000, Proteintech) or rabbit anti-LaminB (1:1000, Proteintech), rabbit anti-thiophosphate ester antibody (1:5000, Abcam), and rabbit anti-phosphoserine (1:500, Abcam). Stripped membranes were probed with a secondary antibody of goat anti-mouse or anti-rabbit IgG (1:10,000, Bioworld) and then visualized with enhanced chemiluminescence.