The content of iridoid compounds was determined in three repetitions according to the method of Duan et al. [11] (link). with own modifications. The mobile phase of the flow rate of 1 mL/min was: H3PO4 (0.1 %) - reagent A and methanol (100 %) - reagent B. The results were recorded at a wavelength of 210 nm. Luna 5 µm C18(2), 250x4.6 mm column (Phenomenex, Torrance, California, USA) and PDA detector were used for chromatographic separation. The gradient program varied linearly: 0–4 min 0–25 % B, 4–10 min 25–35 % B, 10–22 min 35–40 % B, 22–27 min 40–0 % B, 27–42 min 0 % B. The amounts of iridoids were calculated based on standards of loganic acid (ChromaDex), loganin (Sigma-Aldrich), secologanin (ChromaDex) and sweroside (ChromaDex) and the total iridoids (TI) was calculated (mg/100 mL) as a sum of identified iridoids.
Sweroside
Manufactured by ChromaDex
Sourced in United States
Sweroside is a lab equipment product manufactured by ChromaDex. It is a purified compound extracted from plant sources. Sweroside serves as a chemical standard and reference material for analytical testing and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Loganin, by Merck Group (1 mentions)
Luna 5 m c18 2 250x4.6 mm column, by Phenomenex (1 mentions)
Loganic acid, by ChromaDex (1 mentions)
Orthophosphoric acid, by Merck Group (1 mentions)
Isoorientin, by Extrasynthese (1 mentions)
Swertiamarin, by ChromaDex (1 mentions)
Isovitexin, by Extrasynthese (1 mentions)
Isogentisin, by Phytolab (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Gentiopicroside, by ChromaDex (1 mentions)
2 protocols using sweroside
1
Quantitative Analysis of Iridoid Compounds
2
Characterization of Medicinal Plant Compounds
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Maltodextrin (MD) ) was provided from Davisco Foods International (Le Sueur, MN, USA), whey protein (WP) was provided from Polmlek (Raciąż, Poland), pectin was provided from CPKelco (Großenbrode, Germany), and corn starch was supplied from Production sector of the Institute for Medicinal Plants Research Dr. Josif Pančić. Gelatin was produced by Aleva, Novi Kneževac. Folin-Ciocalteu reagent, gallic acid, orthophosphoric acid, and sodium carbonate were purchased from Sigma-Aldrich Chemie GmbH (Munich, Germany). Ultra-pure water was prepared using a Milli-Q purification system (Millipore, France), and HPLC-grade acetonitrile was obtained from Merck (Darmstadt, Germany). Standards swertiamarin, sweroside, and gentiopicroside were purchased from ChromaDex, USA, isoorientin and isovitexin were from Extrasynthese (Cedex, France), and isogentisin was purchased from Phytolab (Germany).
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