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Depletion c2f2

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Depletion; C2F2, is a lab equipment product used for various scientific applications. Its core function is to perform depletion, a process that removes or reduces the presence of specific components within a sample. This product utilizes carbon difluoride (C2F2) as the active agent to carry out the depletion process.

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Lab products found in correlation

Anti ifn γ xmg1, by BioLegend (2 mentions) Anti cd11b m1 70, by BioLegend (2 mentions) Anti cd25 3c7, by BioLegend (2 mentions) Anti cd4 gk1, by BioLegend (2 mentions) Anti cd8a 53 6, by BioLegend (2 mentions) Anti tbet 4b10, by BioLegend (2 mentions) Anti b220 ra3 6b2, by BioLegend (2 mentions) Anti gr 1 rb6 8c5, by BioLegend (2 mentions) Anti foxp3 mf14, by Thermo Fisher Scientific (2 mentions) Anti cd71 r17 217, by BioXCell (2 mentions) Anti cd45 i3 2, by BioLegend (2 mentions) Anti cd90.1 16 10a1, by BioLegend (2 mentions) Anti gzmb gl1, by BioLegend (2 mentions) Anti cxcr5 l138d7, by BioLegend (2 mentions) Anti mouse human cd44 im7, by BioLegend (2 mentions) Anti human mouse bcl 6 7d1, by BioLegend (2 mentions) Anti mouse ki 67 16a8, by BioLegend (2 mentions) Anti mouse tnf α mp6 xt22, by BD (2 mentions) Anti cd3 ucht, by BioLegend (2 mentions) Anti cd8 pra t8, by BioLegend (2 mentions)

2 protocols using depletion c2f2

1

Multicolor Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorophore- or biotin-conjugated antibodies specific for mouse cell-surface antigens and cytokines were as follows: anti-B220 (RA3-6B2, BioLegend), anti-CD4 (GK1.5, BioLegend), anti-CD8a (53-6.7, BioLegend), anti-CD11b (M1/70, BioLegend), anti-Gr1 (RB6-8C5, BioLegend), anti-CD25 (3C7, BioLegend), anti-Foxp3 (MF14, eBioscience), anti-TER119 (TER-119, BioLegend), anti-CD71 (R17 217.1.3, BioXCell for depletion; C2F2, BD biosciences for FACS analysis), anti-CD45 (I3/2.3, BioLegend), anti-CD90.1 (16-10A1, BioLegend), anti-Gzmb (GL1, BioLegend), anti-IFN-γ (XMG1.2, BioLegend). anti-CXCR5 (L138D7, BioLegend), anti-mouse/human CD44 (IM7, BioLegend), anti-human/mouse Bcl-6 (7D1, BioLegend), anti-T-bet (4B10, BioLegend), anti-mouse Ki-67 (16A8, BioLegend), anti-mouse TNF-α (MP6-XT22, BD). For human studies, the following fluorophore- or biotin-conjugated antibodies specific for cell surface markers or cytokines were used: anti-CD3 (UCHT, BioLegend), anti-CD8 (PRA-T8, BioLegend), anti-CD45 (2D1, BioLegend), anti-CD71 (CY1G4, BioLegend), anti-CD235a (HI264, BioLegend). ROS production was measured by labelling with 2′,7′ –dichlorofluorescin diacetate using the ROS detection kit (S0033, Beyotime).
Zhao L., He R., Long H., Guo B., Jia Q., Qin D., Liu S.Q., Wang Z., Xiang T., Zhang J., Tan Y., Huang J., Chen J., Wang F., Xiao M., Gao J., Yang X., Zeng H., Wang X., Hu C., Alexander P.B., Symonds A.L., Yu J., Wan Y., Li Q.J., Ye L, & Zhu B. (2018). Late-stage tumors induce anemia and immunosuppressive extramedullary erythroid progenitor cells. Nature medicine, 24(10), 1536-1544.
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2

Multicolor Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorophore- or biotin-conjugated antibodies specific for mouse cell-surface antigens and cytokines were as follows: anti-B220 (RA3-6B2, BioLegend), anti-CD4 (GK1.5, BioLegend), anti-CD8a (53-6.7, BioLegend), anti-CD11b (M1/70, BioLegend), anti-Gr1 (RB6-8C5, BioLegend), anti-CD25 (3C7, BioLegend), anti-Foxp3 (MF14, eBioscience), anti-TER119 (TER-119, BioLegend), anti-CD71 (R17 217.1.3, BioXCell for depletion; C2F2, BD biosciences for FACS analysis), anti-CD45 (I3/2.3, BioLegend), anti-CD90.1 (16-10A1, BioLegend), anti-Gzmb (GL1, BioLegend), anti-IFN-γ (XMG1.2, BioLegend). anti-CXCR5 (L138D7, BioLegend), anti-mouse/human CD44 (IM7, BioLegend), anti-human/mouse Bcl-6 (7D1, BioLegend), anti-T-bet (4B10, BioLegend), anti-mouse Ki-67 (16A8, BioLegend), anti-mouse TNF-α (MP6-XT22, BD). For human studies, the following fluorophore- or biotin-conjugated antibodies specific for cell surface markers or cytokines were used: anti-CD3 (UCHT, BioLegend), anti-CD8 (PRA-T8, BioLegend), anti-CD45 (2D1, BioLegend), anti-CD71 (CY1G4, BioLegend), anti-CD235a (HI264, BioLegend). ROS production was measured by labelling with 2′,7′ –dichlorofluorescin diacetate using the ROS detection kit (S0033, Beyotime).
Zhao L., He R., Long H., Guo B., Jia Q., Qin D., Liu S.Q., Wang Z., Xiang T., Zhang J., Tan Y., Huang J., Chen J., Wang F., Xiao M., Gao J., Yang X., Zeng H., Wang X., Hu C., Alexander P.B., Symonds A.L., Yu J., Wan Y., Li Q.J., Ye L, & Zhu B. (2018). Late-stage tumors induce anemia and immunosuppressive extramedullary erythroid progenitor cells. Nature medicine, 24(10), 1536-1544.
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