A. marina (Forssk.) Vierh. fruits were collected from Beihai (Guangxi, China). Monosaccharide standards, such as mannose (Man), ribose (Rib), arabinose (Ara), and fucose (Fuc), and bovine serum albumin (BSA), were obtained from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). 3, 5-Dinitrosalicylic acid (DNS) was obtained from Shanghai Solarbio Bioscience & Technology Co., Ltd (Shanghai, China). Lipase (Lot No. SLBL2143V, 41.62 ± 5.62 U/mg determined using triacetin), α-amylase (Lot No. BCCB0496, 30.80 ± 6.32 U/mg), pancreatin (Lot No. SLBV6830, activities of Lipase, amylase, protease in pancreatin were 59.844 ± 10.18, 116.10 ± 12.73, and 135.93 ± 2.11 U/mg, respectively), pepsin (Lot No. BCBR2017V, 301.40 ± 23.98 U/mg), 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP), glucose (Glc), galactose (Gal), galacturonic acid (GalA), glucuronic acid (GlcA), and rhamnose (Rha) were provided by Sigma Chemical Co. (St. Louis, MO, USA). Coomassie brilliant blue G-250, fructooligosaccharide (FOS), trypsin (Lot No. M54318066, 357.35 ± 5.53 U/mL), xylose (Xyl), and amyloglucosidase from Aspergillus niger (Lot No. C10778757, > 100,000 U/mL) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Other commercially obtained chemicals, such as NaCl, NaHCO3 and NaOH, were of analytical grade.
Coomassie brilliant blue g 250
Manufactured by Maclin Biochemical Technology
Sourced in China
Coomassie brilliant blue G-250 is a dye used in protein quantification assays. It binds to proteins, resulting in a color change that can be measured spectrophotometrically to determine protein concentration.
Automatically generated - may contain errors
Lab products found in correlation
Pepsin, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Lipase, by Merck Group (1 mentions)
Rhamnose rha, by Merck Group (1 mentions)
Anhydrous sodium acetate, by Maclin Biochemical Technology (1 mentions)
Ethylene glycol, by Maclin Biochemical Technology (1 mentions)
N n dimethylformamide (dmf), by Maclin Biochemical Technology (1 mentions)
Pancreatic lipase, by Yuanye Bio-Technology (1 mentions)
Zrcl4, by Maclin Biochemical Technology (1 mentions)
Ferric chloride hexahydrate, by Maclin Biochemical Technology (1 mentions)
P nitrophenol, by Maclin Biochemical Technology (1 mentions)
Detergent compatible bradford protein assay kit, by Beyotime (1 mentions)
Vertical mini gel apparatus, by Bio-Rad (1 mentions)
Bovine serum albumin (bsa), by Maclin Biochemical Technology (1 mentions)
L glutathione reduced, by Maclin Biochemical Technology (1 mentions)
4 protocols using coomassie brilliant blue g 250
1
Extraction and Characterization of Mangrove Fruit Polysaccharides
2
Fabrication of Zirconium-based Composite Materials
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Ferric chloride hexahydrate (FeCl3·6H2O; AR, 99%), sodium acrylate (CH2=CHCOONa; 95%), ZrCl4 (99.5%), aminoterephthalic acid (ATA; 98%), N, N-dimethyl-formamide (DMF; AR, 99.5%), ethylene glycol (EG; AR, 98%), anhydrous sodium acetate (CH3COONa; AR, 99%), coomassie brilliant blue G-250 (AR, 98%), p-nitrophenol (AR), and p-nitrophenyl butyrate (p-NPB; 98%) were purchased from Shanghai Macklin Biochemical Co., Ltd., (Shanghai, China). In addition, 50% glutaraldehyde (GA), acetic acid (HAc), ethanol absolute, and ammonium sulfate were of analytical grade and purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., (Shanghai, China). Pancreatic lipase (EC.3.1.1.3 from porcine pancreas) and bovine albumin (BSA; 98%) were provided by Shanghai Yuanye Biotechnology Co., Ltd., (Shanghai, China). S. baicalensis extract was purchased from Beijing Kangrentang Pharmaceutical Co., Ltd., (Beijing, China). All chemicals and reagents were obtained from commercial sources and used without any further purification.
3
SDS-PAGE Analysis of Protein Samples
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SDS-PAGE analysis was performed on a vertical mini-gel apparatus (Bio-Rad, Hercules, United States) at 120 V for 1 h. The resolved protein bands were visualized by staining with Coomassie Brilliant Blue G-250 (Macklin, Shanghai, China) solution. Using bovine serum albumin as the standard, protein concentrations were measured using Detergent Compatible Bradford Protein Assay Kit (Beyotime, Shanghai, China).
4
Analytical Assay of Biochemicals
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All chemicals used are of analytical grade. Bovine serum albumin, 1-chloro-2,4-dinitrobenzene (CDNB), disodium phosphate, Coomassie brilliant blue (G-250), acetylthiocholine iodide (ATChI), and L-glutathione reduced (GSH) were obtained from Macklin Biochemical Co. Ltd (Shanghai, China). 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), phosphate buffer saline (PBS) buffer, and C7-C40n-alkane mixture were acquired from Sigma-Aldrich China Co. Ltd (Shanghai, China). Limonene, (9Z,12Z)-9,12-octadecadienoic acid, n-hexadecanoic acid, and (2E,4E)-2,4-decadienal were purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).
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