One week after the end of the administration of the drug, mice were given 100 μL of pentobarbital sodium salt (70 mg/kg; Nacalai Tesque, Kyoto, Japan) and xylazine hydrochloride (12 mg/kg; Wako Pure Chemical Industries, Osaka, Japan) by intraperitoneal injection, and the trachea was cannulated through an incision in the chest and secured with sterile thread. Then, the forced expiratory volume in 0.05 s (FEV0.05%) was measured using flexiVent (TMSCIREQ©, Montreal, QC, Canada) under the following measurement conditions: ventilator rate, 200/minute; tidal volume, 10 mL/kg; positive end-expiratory pressure, 3 cm H2O; pressure limit, 30 cm H2O.
Xylazine hydrochloride
Manufactured by Fujifilm
Sourced in Japan
Xylazine hydrochloride is a sedative and analgesic compound commonly used in veterinary medicine. It is a central alpha-2 adrenergic agonist that primarily acts on the nervous system to produce its effects. The core function of xylazine hydrochloride is to induce a state of relaxation and reduced sensitivity to pain in animals.
Automatically generated - may contain errors
3 protocols using xylazine hydrochloride
1
Measuring Lung Function in Mice
2
Visualizing Syndecan-1 in Endotoxemia
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Lipopolysaccharides (LPS) from Escherichia coli O26:B6, fluorescein isothiocyanate (FITC)-labeled wheat germ agglutinin (WGA) lectin from Triticum vulgaris, and tetramethylrhodamine (TMR)-labeled dextran (average molecular weight, 75 kDa [TMR-dex75]) were purchased from Sigma-Aldrich Co. (St Louis, MO). The mouse-soluble syndecan-1 (Sdc-1) enzyme-linked immunosorbent assay (ELISA) kit from Diaclone SAS (Besancon Cedex, France) was purchased. Ketamine hydrochloride, xylazine hydrochloride, and rhodamine 6G were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sevoflurane was purchased from Pfizer Japan Inc. (Tokyo, Japan), and used for anesthesia using a small animal anesthetizer (MK-A110D, Muromachi Kikai Co., Ltd., Tokyo, Japan).
3
Anesthesia Protocol for Rat Studies
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Male Lewis rats weighing 200–250 g (Sankyo Labo Service, Tokyo, Japan) were used in the study. All experiments were conducted in accordance with the Animal Care Committee of the Jikei University School of Medicine and the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Rats were anesthetized with a mixture (1:1) of ketamine hydrochloride (10 mg/kg; Wako Pure Chemicals Industries, Osaka, Japan) and xylazine hydrochloride (4 mg/kg; Wako).
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