Microvue bb plus eia kit

Serum Bb level was measured by a MicroVue Bb Plus EIA kit (Quidel). To determine the increase of serum Bb concentration in the presence of cells, 20 µL of NHS was preincubated with 20 µg/mL SARS-CoV-2 spike proteins for 15 minutes on ice, followed by the addition of 80 µL of either GVB⁰ MgEGTA buffer (pH 6.4) or TF1PIGAnull cells (1.2 × 10⁵ cells per sample) in GVB⁰ MgEGTA buffer (pH 6.4). After the reaction was incubated for 15 minutes at 37°C, the cells were centrifuged at 600g for 3 minutes at room temperature and the supernatant was collected for Bb quantification. We also performed the assay with ACH145951 (1.0 µM) and anti-C5Ab (50 µg per sample).