Total protein was extracted from described ovarian cell cultures expressing Caspase 8 or scrambled shRNA untreated, treated with chemotherapy, and/or birinapant using RIPA buffer (sc-24948) according to the manufacturer’s protocol (Santa Cruz Biotechnology, Santa Cruz, CA) and concentrations were estimated with the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis were performed using, respectively, the NuPage system (Invitrogen, Carlsbad, CA) and the Supersignal Chemiluminescent Substrate system (Thermo Scientific, Rockford, IL) using Phospho-ATM (Cell Signaling #4526), Phospho ATR (Abcam #ab227851), and GAPDH (Abcam #ab9485) antibodies.
Phospho atr
Manufactured by Abcam
Sourced in United States
Phospho ATR is a laboratory product used for the detection and quantification of phosphorylated ATR (Ataxia Telangiectasia and Rad3-related) protein. ATR is a serine/threonine-protein kinase that plays a crucial role in the cellular response to DNA damage and replication stress. The Phospho ATR product allows researchers to measure the levels of phosphorylated ATR, which is an important biomarker for various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Nupage system, by Thermo Fisher Scientific (1 mentions)
Phospho atm, by Cell Signaling Technology (1 mentions)
Supersignal chemiluminescent substrate system, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Abcam (1 mentions)
Phospho akt, by Abcam (1 mentions)
Phospho pi3k, by Cell Signaling Technology (1 mentions)
Phospho stat3, by Abcam (1 mentions)
Phospho stat1, by Abcam (1 mentions)
Phospho atm, by Abcam (1 mentions)
Stat1, by Abcam (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Annexin 5 propidium iodide apoptosis detection kit, by BD (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Mcl 1, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Stat3, by Abcam (1 mentions)
Jc 1 kit, by Beyotime (1 mentions)
2 protocols using phospho atr
1
Phospho-ATM and Phospho-ATR Detection
2
Evaluating Apoptosis Signaling Pathways
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DCZ0014 stock solution was dissolved in dimethyl sulfoxide (DMSO; Sigma, St. Louis, MO, USA) and stored at - 20 °C. Antibodies for phospho-PI3K, cleaved caspase-3, cleaved Caspase-8, Caspase-9, poly ADP-ribose polymerase (PARP), Bax, Bad, B cell lymphoma-2 (Bcl-2), Bcl-xl, C-myc, Mcl-1 and β-actin (for western blot) were purchased from Cell Signaling Technology (Danvers, MA, USA). Lyn, phospho-Lyn, Syk, phospho-Syk, Akt, phospho-Akt, STAT3, phospho-STAT3, STAT1, phospho-STAT1, ATM, phospho-ATM, ATR, phospho-ATR, CHK2, phospho-checkpoint kinase2 (p-CHK2), CHK1, phospho-checkpoint kinase1 (p-CHK1), cell division cycle 25A (cdc25A), CDK4, CDK6 and cyclinD1 antibodies were obtained from Abcam (Cambridge, MA, USA). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan), the Annexin-V/ propidium iodide (PI) apoptosis detection kit from BD Pharmingen (Franklin Lakes, NJ, USA) and the JC-1 Kit from Beyotime Institute of Biotechnology (Haimen, China).
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