Data acquisition was performed as indicated using either a four-laser FACSAria II cell sorter (no UV laser), a FACSAria Fusion cell sorter, or a BD LSRII analytical cytometer (BD Biosciences) each equipped with five lasers: UV (355 nm), violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (637 nm). Cytometer calibration and control fluorescent measurements were performed using SPHERO eight-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL). Compensation was calculated using FACS DIVA Software (BD Biosciences) acquisition-defined matrices. Data were analyzed with FlowJo 10 (TreeStar, Ashland, OR).
Lsrii analytical cytometer
Manufactured by BD
The BD LSRII is an analytical cytometer designed for flow cytometry applications. It is equipped with multiple laser sources and detectors to analyze the characteristics of individual cells or particles flowing through the system. The core function of the BD LSRII is to provide high-performance cell analysis and sorting capabilities for researchers and laboratory professionals.
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2 protocols using lsrii analytical cytometer
1
Multiparameter Flow Cytometry Workflow
2
Multiparameter Flow Cytometry Workflow
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Data acquisition was performed as indicated using either a four-laser FACSAria II cell sorter (no UV laser), a FACSAria Fusion cell sorter, or a BD LSRII analytical cytometer (BD Biosciences) each equipped with five lasers: UV (355 nm), violet (405 nm), blue (488 nm), yellow-green (561 nm), and red (637 nm). Cytometer calibration and control fluorescent measurements were performed using SPHERO eight-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL). Compensation was calculated using FACS DIVA Software (BD Biosciences) acquisition-defined matrices. Data were analyzed with FlowJo 10 (TreeStar, Ashland, OR).
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