Ccd 18co normal colon fibroblast cell line

The human colorectal cancer cell lines used in this study were purchased from ATCC (Manassas, VA). The CCD-18Co normal colon fibroblast cell line was purchased from the Korea Cell Line Bank (KCLB) (Seoul, Korea). The HCT15, SW620, SW480, SW48, LoVo, and DLD-1 cells were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. The HT29 and HCT116 cells were maintained in McCoy’s 5A medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. The CCD-18Co cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. All cells were maintained at 37°C in a 5% CO₂ humidified incubator. The siRNA was transfected using Lipofectamine 2000 reagent (Invitrogen). The sense sequence of the Ino80-specific siRNA was 5′-uuaagagugugauuucucauu.