Spectra x light

Manufactured by Lumencor

The Spectra X light is a light-emitting device designed for laboratory applications. It provides a versatile and adjustable source of illumination across a wide range of wavelengths, enabling users to customize the light output to meet their specific experimental needs.

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Lab products found in correlation

Axio observer microscope, by Zeiss (1 mentions) Axiovision, by Zeiss (1 mentions) Intensilight, by Nikon (1 mentions) Ti inverted, by Nikon (1 mentions) Metamorph, by Molecular Devices (1 mentions)

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2 protocols using spectra x light

Optogenetic Stimulation of Neuronal Proteins

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Optogenetic stimulation was performed using an external light source (Lumencor spectra X light, spectral output 100% = 310 mW/nm) directed towards the craniotomy. NpHR is activated by yellow light (575 nm, continuous stimulation, 5 mW). SwiChR++ is opened by cyan (470 nm, 5 s, 5 mW) light and closed by red (600 nm, 5 s, 5 mW) light.

Untiet V., Beinlich F.R., Kusk P., Kang N., Ladrón-de-Guevara A., Song W., Kjaerby C., Andersen M., Hauglund N., Bojarowska Z., Sigurdsson B., Deng S., Hirase H., Petersen N.C., Verkhratsky A, & Nedergaard M. (2023). Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice. Nature Communications, 14, 1871.

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Fluorescence and Mass Microscopy Protocol

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Acquisitions were performed on a Ti inverted (Nikon) or an Axio Observer microscope (Carl Zeiss) at 37°C, with 5% CO₂ atmosphere, using a 10× dry objective (NA 0.30 phase), a 20× dry objective (NA 0.5 phase), or a SFluor 20× objective (NA 0.75 without phase ring) for the mass measurements. Images were acquired using MetaMorph (Molecular Devices) or Axio Vision (Carl Zeiss) software. The excitation source was either a mercury lamp (Intensilight; Nikon) or a LED illumination (SPECTRA X light; Lumencor or Zeiss Colibri). Images were acquired with a CoolSNAP HQ2 camera (Photometrics) for fluorescence or a PHASICS camera (PHASICS) for mass measurements.

Zlotek-Zlotkiewicz E., Monnier S., Cappello G., Le Berre M, & Piel M. (2015). Optical volume and mass measurements show that mammalian cells swell during mitosis. The Journal of Cell Biology, 211(4), 765-774.

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