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Fitc filter set 38 he

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The FITC (Filter Set 38 HE) is a filter set designed for fluorescence microscopy applications. It is optimized for the detection of fluorescein isothiocyanate (FITC) fluorescent labels. The filter set includes an excitation filter, a dichroic mirror, and an emission filter to isolate the FITC fluorescence signal.

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Lab products found in correlation

Axio observer z1 apotome fluorescence microscope, by Zeiss (2 mentions) Metafer, by MetaSystems (2 mentions)

Close spelling variants of this product

Filter Set 38 HE (13 citations) Filter Set 38 (10 citations)

2 protocols using fitc filter set 38 he

1

Automated Screening of Fusion Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Zeiss Axio Observer z1 Apotome fluorescence microscope was used for the imaging and scoring of mFISH samples. The microscope was set up with commercially available DAPI (Filter Set 49, 488049-9901-000) and FITC (Filter Set 38 HE, 489038-9901-000) filters purchased from Zeiss, and of custom-made DEAC (49302), Texas Red (49008), GOLD (49034), FITC/TXR (59022) filters purchased from Chroma. Slides were scanned overnight using Metafer (MetaSystems), an automated high-throughput software specialized in FISH images acquisition and processing. For each sample, 100-600 interphase nuclei were scanned, and 100-250 cells manually scored for the presence of the ETV6–RUNX1 fusion gene in combination with deletion (hemizygous or homozygous) or amplification of ETV6 (TEL), RUNX1 (AML1), PAX5 and CDKN2A (p16).
Turati V.A., Guerra-Assunção J.A., Potter N.E., Gupta R., Ecker S., Daneviciute A., Tarabichi M., Webster A.P., Ding C., May G., James C., Brown J., Conde L., Russell L.J., Ancliff P., Inglott S., Cazzaniga G., Biondi A., Hall G.W., Lynch M., Hubank M., Macaulay I., Beck S., Van Loo P., Jacobsen S.E., Greaves M., Herrero J, & Enver T. (2021). Chemotherapy induces canalization of cell state in childhood B-cell precursor acute lymphoblastic leukemia. Nature cancer, 2(8), 835-852.
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2

Automated Screening of Fusion Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Zeiss Axio Observer z1 Apotome fluorescence microscope was used for the imaging and scoring of mFISH samples. The microscope was set up with commercially available DAPI (Filter Set 49, 488049-9901-000) and FITC (Filter Set 38 HE, 489038-9901-000) filters purchased from Zeiss, and of custom-made DEAC (49302), Texas Red (49008), GOLD (49034), FITC/TXR (59022) filters purchased from Chroma. Slides were scanned overnight using Metafer (MetaSystems), an automated high-throughput software specialized in FISH images acquisition and processing. For each sample, 100-600 interphase nuclei were scanned, and 100-250 cells manually scored for the presence of the ETV6–RUNX1 fusion gene in combination with deletion (hemizygous or homozygous) or amplification of ETV6 (TEL), RUNX1 (AML1), PAX5 and CDKN2A (p16).
Turati V.A., Guerra-Assunção J.A., Potter N.E., Gupta R., Ecker S., Daneviciute A., Tarabichi M., Webster A.P., Ding C., May G., James C., Brown J., Conde L., Russell L.J., Ancliff P., Inglott S., Cazzaniga G., Biondi A., Hall G.W., Lynch M., Hubank M., Macaulay I., Beck S., Van Loo P., Jacobsen S.E., Greaves M., Herrero J, & Enver T. (2021). Chemotherapy induces canalization of cell state in childhood B-cell precursor acute lymphoblastic leukemia. Nature cancer, 2(8), 835-852.
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