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3 protocols using isopropanol

1

Analytical Reagent Procurement for Research

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HPLC grade methanol, acetonitrile, and isopropanol were obtained from Chem-Lab (Zedelgem, Belgium), while phenol and GABA standards were obtained from Sigma Aldrich (St. Louis, MO, USA). Analytical grade anhydrous ethanol and sodium hydroxide were purchased from Thermo Fisher Scientific (Waltham, MA, USA). GO standards were obtained from TCI Europe (Zwijndrecht, Belgium).
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2

Protein-based Bioelectronic Sensor Development

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Human α-synuclein, glutaraldehyde (25% wt), potassium chloride, sodium phosphate dibasic, iron(III) ferrocyanide, polystyrene (MW 10000 g/mol), chlorobenzene, 2,3,4,5,6pentafluorothiophenol, dextran (from Leuconostoc Mesenteroides, MW= 64.000-76.000 g/mol), and gold wire (Ø 0.5 mm) were purchased from Sigma Aldrich. Sodium chloride and glycerol were purchased from Panreac Quimica. Recombinant His-tagged Protein G was obtained from BioVision, Inc. HS-(CH2)11-(OCH2CH2)6-NH2•HCl (abbreviated as HSC11EG6NH2) was purchased from ProChimia Surfaces. The anti-(α-synuclein) antibody α-synuclein(211) was purchased from Santa Cruz Biotechnology, Inc. The organic semiconductor, 2,8-Difluoro-5,11bis(triethylsilylethynyl)anthradithiophene (diF-TES-ADT), was obtained from Lumtec. Acetone and isopropanol were purchased from Chem-Lab and used without further purification. Kapton ® foils were bought from DuPont (Kapton ® HN, 75 μm thick). Qsil216 A/B (polydimethylsiloxane, PDMS) was purchased from Farnell Componentes.
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3

Sensitizing Chemicals Evaluation Protocol

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Sensitizing chemicals were benzylideneacetone (CAS 122-57-6, Sigma), oxazolone (15646-46-5, Sigma), 1,4-benzoquinone (CAS 106-51-4, Sigma), 2,4-dinitrochlorobenzene (CAS 97-00-7, Sigma), 4-nitrobenzylbromide (CAS 100-11-8, Sigma), glyoxal (CAS 107-22-2, Sigma), methyldibromoglutaronitrile (CAS 35691-65-7, Sigma), cinnamic aldehyde (CAS 104-55-2, SAFC), isoeugenol (CAS 97-54-1, Sigma), ethylene glycol dimethacrylate (CAS 97-90-5, Sigma), 2-mercaptobenzothiazole (CAS 149-30-4, Sigma), tetramethyl thiuram disulphide (CAS 137-26-8, Sigma), eugenol (CAS 97-53-0, Sigma), cinnamic alcohol (CAS 104-54-1, Sigma) and non-sensitizing substances include sodium dodecyl sulfate (CAS 151-21-3, Carl Roth), salicylic acid (CAS 69-72-7, Carl Roth), glycerol (CAS 56-81-5, Carl Roth), lactic acid (CAS 50-21-5, Carl Roth), methyl salicylate (CAS 119-36-8, Thermofisher), diethylphthalate (CAS 84-66-2, Thermofisher), sulphanilamide (CAS 64-74-1, Thermofisher), chlorobenzene (CAS 108-90-7, VWR) and isopropanol (67-63-0, CHEM-LAB).
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