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Automated thermoregulation system

Manufactured by Kopf Instruments
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The Automated Thermoregulation System is a laboratory equipment that precisely controls and monitors the temperature of samples or experiments. It maintains a stable and consistent temperature environment to support various research and testing applications.

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Lab products found in correlation

2 protocols using automated thermoregulation system

1

Stereotaxic Transplantation of HuCNS-SC in Mice

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For transplantation surgeries, mice were anesthetized with isoflurane (Western Medical Supply, CA), and placed into a Kopf stereotaxic frame. Normal body temperature was maintained using an automated thermoregulation system (KOPF Instruments, CA). HuCNS‐SC, at 1.0 × 105 cells per site (2 μL/injection), or vehicle, were injected bilaterally into the hippocampus at a rate of 1 µL/min using the following coordinates relative to bregma: anteroposterior (A/P): −2.06 mm; dorsoventral (D/V): −1.95 mm; mediolateral (M/L): ±1.75 mm. Transplantation of HuCNS‐SC into 3xTg‐AD mice used identical procedures and coordinates. After surgery, the incision was sealed with Tissuemend II (Western Medical Supply, CA) and topical antibiotic ointment applied before allowing mice to recover on heated pads.
Ager R.R., Davis J.L., Agazaryan A., Benavente F., Poon W.W., LaFerla F.M, & Blurton‐Jones M. (2015). Human neural stem cells improve cognition and promote synaptic growth in two complementary transgenic models of Alzheimer's disease and neuronal loss. Hippocampus, 25(7), 813-826.
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2

Stereotaxic Transplantation of HuCNS-SC in Alzheimer's Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
For transplantation surgeries, mice were anesthetized with isoflurane (Western Medical Supply, CA), and placed into a Kopf stereotaxic frame. Normal body temperature was maintained using an automated thermoregulation system (KOPF Instruments, CA). HuCNS-SC, at 1.0 × 105 cells per site (2 µL/injection), or vehicle, were injected bilaterally into the hippocampus at a rate of 1 µL/min using the following coordinates relative to bregma: anteroposterior (A/P): −2.06 mm; dorsoventral (D/V): −1.95 mm; mediolateral (M/L): ± 1.75 mm. Transplantation of HuCNS-SC into 3xTg-AD mice used identical procedures and coordinates. After surgery, the incision was sealed with Tissuemend II (Western Medical Supply, CA) and topical antibiotic ointment applied before allowing mice to recover on heated pads.
Ager R.R., Davis J.L., Agazaryan A., Benavente F., Poon W.W., LaFerla F.M, & Blurton‐Jones M. (2015). Human neural stem cells improve cognition and promote synaptic growth in two complementary transgenic models of Alzheimer's disease and neuronal loss. Hippocampus, 25(7), 813-826.
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