Immortalized mouse hippocampal cell line, HT-22 cell line, which is a sub-line derived from parent HT4 cells that were originally immortalized from primary mouse hippocampal neuron culture (Lonza, Walkersville, MD) was grown in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 25 mM glucose, 2 mM L-glutamine, 2 mM pyruvate, penicillin (10 units/ml), streptomycin (10 mg/ml), and 10% (vol/vol) heat inactivated FBS in a humidified cell incubator (Binder, Germany) at 37°C under a 5% CO2 atmosphere. Primary hippocampal neuron cultures were prepared from embryonic day 18–19 (E18-19) pregnant Sprague-Dawley rats by dissociating with 0.25% trypsin and plated onto coverslips coated with poly-L-lysine. Neurons were grown in neurobasal medium supplemented with B27, 2mM GlutaMAX-I supplement and 100 μg/ml penicillin/streptomycin at 37°C in a humidified environment of 95% O2/5% CO2.
Humidified cell incubator
Manufactured by Binder
Sourced in Germany
The Humidified Cell Incubator is a laboratory equipment designed to provide a controlled and stable environment for cell culture applications. Its core function is to maintain optimal temperature and humidity levels to support the growth and maintenance of cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using humidified cell incubator
1
Mouse Hippocampal Cell Culture Protocol
2
Isolation of Primary Astrocyte Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary astrocyte culture was described previously [15] . Briefly, primary mixed glial cultures were prepared from postnatal day 1 (P1) C57B/L6 mice. The cortex was dissociated with 0.25% trypsin and filtered with a cell strainer. The mixed glia was grown in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% (vol/vol) heat-inactivated FBS and penicillin (10 units/ml) and streptomycin (10 mg/ml) in a humidified cell incubator (Binder, Germany) at 37°C under a 5% CO 2 atmosphere. When the mixed glia filled 80% of the T75 plate, astrocyte and microglia were isolated via a 250 rpm shaking incubator at 37°C for overnight. Microglia were detached from the plate after shaking isolation. The astrocytes were detached with 0.25% trypsin-EDTA and seeded onto poly-L-lysine coated plates for the experiment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!