The morphologies of MAL-PEG-b-PLL/PBA, PMs@NaF and PMs@NaF-SAP were observed using transmission electron micrographs (TEM, JEM-100SX, Japan). The particle size and zeta potential of polymers were measured using dynamic light scattering (DLS, Zetasizer Nano ZS90, Malvern Instruments, Malvern, UK). The concentrations of each polymer were measured by lyophilization. The molecular weight and polydispersity index (PDI) of the samples were determined by Gel Permeation Chromatography (GPC, Agilent PL-GPC50/Agilent 1260). The FT-IR spectra of the samples were recorded using a fourier transform infrared spectrometer (FTIR spectrometer, NICOLET 5700) to confirm the successful conjugation between PMs@NaF and SAP. After dissolving in DMSO-d6, the 1H NMR spectra of MAL-PEG-b-PLL/PBA and PMs were recorded using a Bruker Avance spectrometer (Bruker AV II-400 MHz).
Pl gpc50 agilent 1260
Manufactured by Agilent Technologies
The PL-GPC50/Agilent 1260 is a gel permeation chromatography (GPC) system designed for the analysis of polymer molecular weight and molecular weight distribution. It features an Agilent 1260 Infinity II LC system with a refractive index (RI) detector.
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2 protocols using pl gpc50 agilent 1260
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Characterization of Polymeric Nanoparticles
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1,1-Carbonyldiimidazole (CDI), branched polyethylenimine (PEI, Mw = 800 Da), and folic acid were purchased from Sigma-Aldrich. GdCl3·6H2O was purchased from Tokyo Chemical Industry. N3-PEG2000-NH2 was obtained from Xiamen Sinopeg Biotech CO., Ltd. Cyclodextrin underwent recrystallization three times in water, and was stored in a vacuum drying oven at 60 °C before use. DBCO-PEG2000-NH2 was purchased from Xi'an Confluore Biological Technology Co., Ltd. Ad-PEG2000-PLL(DTPA-Gd)-N3 and DBCO-PEG2000-FA were synthesized in our lab.30 All the other reagents were obtained from the domestic suppliers.
1H NMR spectra were obtained using a Varian NMR spectrometer at 400 MHz. Gel permeation chromatography (GPC) measurements were carried out using an Agilent PL-GPC50/Agilent 1260, 0.15 M NaNO3 water solution was used as an eluent with a flow rate of 1 mL min−1. The Gd concentrations of the samples were detected via an inductively coupled plasma optical emission spectrometer (ICP-OES, Agilent 720ES). The in vitro T1-weighted data were obtained via a 0.5T NMR-analyzer, while the in vivo T1-weighted MR images were acquired on a 4.7T scanner (Bruker).
1H NMR spectra were obtained using a Varian NMR spectrometer at 400 MHz. Gel permeation chromatography (GPC) measurements were carried out using an Agilent PL-GPC50/Agilent 1260, 0.15 M NaNO3 water solution was used as an eluent with a flow rate of 1 mL min−1. The Gd concentrations of the samples were detected via an inductively coupled plasma optical emission spectrometer (ICP-OES, Agilent 720ES). The in vitro T1-weighted data were obtained via a 0.5T NMR-analyzer, while the in vivo T1-weighted MR images were acquired on a 4.7T scanner (Bruker).
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