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Anti itgb3 cd61 pe antibodies

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Anti-ITGB3/CD61-PE antibodies are fluorochrome-conjugated antibodies that specifically bind to the ITGB3/CD61 antigen. They are designed for use in flow cytometry and other immunoassays to detect and analyze cells expressing the ITGB3/CD61 protein.

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Lab products found in correlation

96 well clear round bottom ultra low attachment microplate, by Corning (2 mentions) Aria 2, by BD (2 mentions) Anti ssea1 cd71 efluor660, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

CD61-PE (7 citations) Anti‐CD61 (4 citations) ITGB3 (3 citations)

2 protocols using anti itgb3 cd61 pe antibodies

1

Generation of PGCLCs from ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
1.5×105 cell/well of ESCs were plated on a 12-well plate and induced into EpiLCs for 48 h as described previously43 (link). The EpiLCs were then cultured under a floating condition by plating 2–3×103 cells per well of a 96-Well Clear Round Bottom Ultra Low Attachment Microplate (Corning, cat#7007) in GK15 medium supplemented with LIF, BMP4 (500 ng/mL), SCF (100 ng/mL), and EGF (50 ng/mL). d4 PGCLCs were purified with a fluorescence-activated cell sorter (FACS) (ARIA II; BD Biosciences) by using anti-SSEA1/CD71-eFluor660 (1:20 dilution; eBioscience, cat #50-8813-41) and anti-ITGB3/CD61-PE antibodies (1:200 dilution; BioLegend, cat #104307).
Yamaji M., Jishage M., Meyer C., Suryawanshi H., Der E., Yamaji M., Garzia A., Morozov P., Manickavel S., McFarland H.L., Roeder R.G., Hafner M, & Tuschl T. (2017). DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature, 543(7646), 568-572.
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2

Generation of PGCLCs from ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
1.5×105 cell/well of ESCs were plated on a 12-well plate and induced into EpiLCs for 48 h as described previously43 (link). The EpiLCs were then cultured under a floating condition by plating 2–3×103 cells per well of a 96-Well Clear Round Bottom Ultra Low Attachment Microplate (Corning, cat#7007) in GK15 medium supplemented with LIF, BMP4 (500 ng/mL), SCF (100 ng/mL), and EGF (50 ng/mL). d4 PGCLCs were purified with a fluorescence-activated cell sorter (FACS) (ARIA II; BD Biosciences) by using anti-SSEA1/CD71-eFluor660 (1:20 dilution; eBioscience, cat #50-8813-41) and anti-ITGB3/CD61-PE antibodies (1:200 dilution; BioLegend, cat #104307).
Yamaji M., Jishage M., Meyer C., Suryawanshi H., Der E., Yamaji M., Garzia A., Morozov P., Manickavel S., McFarland H.L., Roeder R.G., Hafner M, & Tuschl T. (2017). DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature, 543(7646), 568-572.
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