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Orca c13440

Manufactured by Hamamatsu Photonics

The ORCA C13440 is a high-performance scientific CMOS (sCMOS) camera developed by Hamamatsu Photonics. It features a large effective pixel area, low noise, and high quantum efficiency, making it suitable for a variety of scientific imaging applications.

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2 protocols using orca c13440

1

Imaging Lysosomal Markers in C. elegans

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The epidermal lysosomal marker (PCED-1::nuc-1::mCherry)43 (link), the pan-neuronal marker (PRGEF-1::DsRed)54 (link), and the PVD/AQR-neuronal marker (PF49H12.4::GFP from NC1687) were imaged after worms were mounted on Noble agar pads in M9 with NaN3 (0.25%). Zeiss AxioImager M2 with a black and white Hamamatsu ORCA C13440 camera was used for DIC images. All images were taken at the same magnification for the same amount of time for comparisons as indicated in the relevant figure legends. Fiji (Image J) was used for lysosome quantification. The same threshold was applied to all mutants. For lysosomal structure, puncta with circularity of 0.4 to 1 were quantified as particles and puncta with circularity of 0–0.4 were quantified as tubules.
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2

Comparative Imaging of Biological Samples

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Images were taken with either a Leica MZ 16F dissecting scope and a Hamamatsu ORCA-ER C4742-95 digital camera (Figures 1, 2 and 3), a Zeiss Axiozoom V16 dissecting scope with a Zeiss Axiocam 506 mono camera (Figures 4 and 5), a fluorescent microscope with DIC (Nomarski) optics (Zeiss Axioplan 2) with a Zeiss Axiocam MRm (Figure 2), or a Zeiss AxioImager M2 with a Hamamatsu ORCA C13440 camera (Figure 5). All images were taken at the same magnification for the same amount of time for comparisons as indicated in the relevant figure legends.
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