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Escherichia coli e coli atcc 8739

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Escherichia coli (E. coli) ATCC 8739 is a bacterial strain obtained from the American Type Culture Collection. It is a Gram-negative, rod-shaped bacterium commonly found in the lower intestine of warm-blooded organisms, including humans. This strain is often used as a reference or control culture in various microbiological and biochemical applications.

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4 protocols using escherichia coli e coli atcc 8739

1

Antimicrobial Polymer Synthesis and Evaluation

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Rifampicin (MW 822.95 amu) was purchased from TGI (Tokyo, Japan), cefixime trihydrate (MW 507.5 amu) was kindly donated by Dar Al Dawa Pharmaceuticals (Amman, Jordan). 2-Hydroxyethyl methacrylate (2-HEMA) (MW 130.14 amu), ethylene glycol di methacrylate (EGDMA) (MW 198.22 amu), 2.2-Azobis (2-methyl-propionitrile) (AIBN) (MW 164.21 amu) were obtained from Sigma-Aldrich (Munich, Germany). Bacteria were obtained from American Type Culture Collection Organization (Manassas, Virginia, USA); Staphylococcus aureus (S. aureus) ATCC 6538, Escherichia coli (E. coli) ATCC 8739, and Pseudomonas aeruginosa (P. aeruginosa) ATCC 9027. Brain Heart Infusion broth (BHI) was purchased from biolab (Budapest, Hangary). Müller Hinton agar and Nutrient Broth (NB) obtained from HIMEDIA (Mumbai, India). Human embryonic kidney (HEK) cell line from ATCC (USA).
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2

Antimicrobial Evaluation of Bacterial Strains

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The Gram-positive bacteria (Bacillus spizizenii (B. spizizenii) ATCC 6633, Staphylococcus aureus (S. aureus) ATCC 6538), and Gram-negative bacteria (Escherichia coli (E. coli) ATCC 8739, Pseudomonas aeruginosa (P. aeruginosa) ATCC 12453, and Proteus mirabilis (P. mirabilis) ATCC 43071) used in this study were obtained from the American Type Culture Collection (ATCC). The bacterial strains were grown at 37 °C and maintained on nutrient agar.
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3

Antimicrobial Activity of Functionalized Albumin

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Bovine serum albumin (BSA), polyoxyethylene (10) cetyl ether (Brij 58), rhodamine, Thioflavin T (ThT), rapamycin, thiazolyl blue tetrazolium bromide, and paraformaldehyde were purchased from Sigma-Aldrich. SA was purchased from Aladdin. Paclitaxel was purchased from Macklin. Phosphate buffered saline (PBS), Mueller–Hinton broth (MHB), Mueller–Hinton agar (MHA), and methyl blue (MB) were purchased from Solarbio. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) was purchased from TCI. Dimethyl sulfoxide (DMSO), ethanol, acetonitrile, 30% hydrogen peroxide (H2O2), sodium hydroxide (NaOH), sulfuric acid (H2SO4), and Tween 80 were purchased from Sinopharm Chemical Reagent Co., Ltd. 5(6)-aminofluorescein-labeled SA was purchased from Ruixi Biological Technology Co., Ltd. Staphylococcus aureus (S. aureus) (ATCC6538) and Escherichia coli (E. coli) (ATCC8739) were obtained from American Type Culture Collection (USA). The silicone urinary catheter was purchased from Wellead Medical Instruments Co., Ltd. (Guangzhou, China).
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4

Bacterial Strain Cultivation for Antibiotic Testing

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The Gram-positive bacteria (Staphylococcus aureus (S. aureus) ATCC 6538) and Gram-negative bacteria (Escherichia coli (E. coli) ATCC 8739) used in this study were obtained from the American Type Culture Collection (ATCC). The bacterial strains were grown at 37 °C and maintained on nutrient agar.
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