Femoral and tibial bone marrow was isolated as described (Schulze, 2016 ). Bone-marrow cells were stained with FITC-conjugated rat anti-mouse CD41 or rat-IgG isotype control (both from BD Biosciences). Cells were washed in phosphate buffered saline (PBS) followed by fixation with 4% paraformaldehyde at room temperature for 1 h. After washing twice with PBS, cells were incubated with 5 μg/ml of DAPI and analyzed by flow cytometry (Trakala et al., 2015 (link)). HEL cells were stained with FITC-conjugated mouse anti-human CD41 (BD Biosciences), followed by flow cytometry analysis as above.
Rat igg isotype control
Manufactured by BD
Rat-IgG isotype control is a laboratory reagent used in flow cytometry and other immunoassays to establish background staining levels. It serves as a control to differentiate specific antibody binding from non-specific binding.
Automatically generated - may contain errors
Lab products found in correlation
Fitc conjugated mouse anti human cd41, by BD (1 mentions)
Fitc conjugated rat anti mouse cd41, by BD (1 mentions)
Acldl, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 goat anti rat, by Thermo Fisher Scientific (1 mentions)
Rat anti mouse cd31, by BD (1 mentions)
Apc cy7 rat anti mouse cd45, by BD (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Goat anti rabbit fitc, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rat igg isotype control
1
Immunophenotyping of Hematopoietic Cells
2
Multicolor Flow Cytometry Analysis
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Selected antibodies were acquired as indicated: Rat IgG Isotype control (553993, BD Pharmingen), Rat IgG Fluorescent Isotype controls (sc-2831, sc-3788, sc-2895, sc 2872; Santa Cruz Biotechnology), Rat Anti-Mouse Flk-1-PE (555308, BD Pharmingen), Rat Anti-Mouse c-Kit-PE-Cy7 (558163, BD Pharmingen), Rat Anti-Mouse CD45-APC-Cy7 (557659, BD Pharmingen), Rat Anti-Mouse CD31-FITC (553372, BD Pharmingen), Mouse Anti-Human VEGFR2-PE (560872, BD Pharmingen), Mouse Anti-Human CD117-APC (561118, BD Pharmingen), Mouse Anti-Human CD45-APC-Cy7 (557833, BD Pharmingen), Mouse Anti-Human CD31-FITC (560984, BD Pharmingen), Rat Anti-Mouse CD31 (550274, BD Pharmingen), Goat Anti-Rat FITC (sc-2011, Santa Cruz Biotechnology), Goat Anti-Rat AlexaFluor594 (A-11007, Life Technologies), Anti-Rabbit Oct-4 (AB3209, EMD Millipore), and Goat Anti-Rabbit FITC (sc-2012, Santa Cruz Biotechnology). Additional reagents used: Hoechst’s 33258 Stain (94403, Sigma-Aldrich), acLDL-Dil (L3484, Life Technologies), acLDL-BODIPY (L3485, Life Technologies), acLDL (L35354, Life Technologies), Fibronectin (F1141, Sigma-Aldrich), and Alkaline Phosphatase Stain (A14353, Life Technologies).
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