For immunophenotype studies, dual-color immunofluorescence was performed using the following panel of phycoerythrin (PE)-conjugated or fluorescein isothiocyanate (FITC)-conjugated monoclonal antibodies: anti-human CD14, anti-human CD13, anti-human HLA-DR, anti-human CD34, and anti-human CD73 (BD Biosciences, Buccinasco, Italy); and anti-human CD105, anti-human CD44, anti-human CD45, anti-human CD90, and anti-human CD29 (Chemicon, Temecula, CA, USA). Negative controls were isotype-matched irrelevant monoclonal antibodies (BD Biosciences). MSCs (5 × 105) were incubated in the dark for 15 minutes at 4 °C in PBS–1 % bovine serum albumin (BSA). Cells were rinsed in PBS and analyzed using a BD Accuri C6 flow cytometer (BD Biosciences). A minimum of 10,000 events was collected in list mode on FCS Express 4 Flow Research Edition Software (De Novo software, Glendale, CA, USA).
Isotype matched irrelevant monoclonal antibodies
Manufactured by BD
Sourced in United States
Isotype-matched irrelevant monoclonal antibodies are laboratory reagents used as control samples in immunoassays and other applications. They are antibodies that are of the same isotype as the primary antibody being used, but are specific to an antigen not present in the sample being tested. These control antibodies help establish baseline signals and account for non-specific binding in the assay.
Automatically generated - may contain errors
Lab products found in correlation
Anti human hla dr, by BD (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
Fcs express 4 flow research edition software, by De Novo Software (1 mentions)
Anti human cd73, by BD (1 mentions)
Anti human cd90, by Merck Group (1 mentions)
Anti human cd13, by BD (1 mentions)
Facscalibur machine, by BD (1 mentions)
Dissociation solution, by Merck Group (1 mentions)
2 protocols using isotype matched irrelevant monoclonal antibodies
1
Immunophenotyping of Mesenchymal Stem Cells
2
Characterization of Cell Surface Markers
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Isolated cells were harvested using dissociation solution (Sigma, USA), washed twice with PBS and then stained with fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD45, CD34 and CD14 (BD Biosciences, USA) and phycoerythrin (PE)-conjugated mouse anti-human CD44 and CD166 (BD Biosciences, USA) and APC-conjugated mouse anti-human CD73. Isotype-matched irrelevant monoclonal antibodies (BD-Pharmingen, USA) were used as negative controls. After 30 minutes incubation at room temperature and washing with PBS, approximately 10,000 events were collected on a FACS Calibur machine (BD Biosciences, USA) using the Cell quest as data acquisition software and analyzed using FlowJo7.6 software for the graphical presentation of data.
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