Hrp conjugated anti mouse β actin antibody

Manufactured by Merck Group

The HRP-conjugated anti-mouse β-actin antibody is a laboratory reagent used for the detection and quantification of the β-actin protein in mouse samples. It is a primary antibody conjugated with horseradish peroxidase (HRP) enzyme, which enables it to be used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry.

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2 protocols using hrp conjugated anti mouse β actin antibody

Western Blot Analysis of TNIK and β-Actin

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Total cellular extracts from organ lysates were prepared in lysis buffer supplemented with proteinase inhibitor (Roche) and phosphatase inhibitor (Thermo Scientific). Protein samples were denaturated at 95 °C for 5 min and loaded on Mini-proteanTGX precast gels (BioRad) for SDS-PAGE. The gel was blotted onto the polyvinylidene difluoride membrane (BioRad). After incubation with the primary polyclonal rabbit-α-mouse TNIK antibody (Thermo Scientific) at 4 °C overnight, horseradish peroxidase (HRP)-conjugated goat-anti-rabbit IgG (Thermo Scinentific) was used for primary antibody labeling. Alternatively, monoclonal HRP-conjugated anti-mouse β-actin antibody was used (SIGMA). Specifically labeled protein bands were detected with Clarity Western ECL Blotting Substrate using enhanced chemiluminescence (BioRad).

Jaeger-Ruckstuhl C.A., Hinterbrandner M., Höpner S., Correnti C.E., Lüthi U., Friedli O., Freigang S., Al Sayed M.F., Bührer E.D., Amrein M.A., Schürch C.M., Radpour R., Riether C, & Ochsenbein A.F. (2020). TNIK signaling imprints CD8+ T cell memory formation early after priming. Nature Communications, 11, 1632.

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Comprehensive Antibody Characterization Protocol

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Commercially available antibodies were obtained as follows: anti-caspase 12, anti-caspase 3, anti-caspase 9, anti-cleaved caspase 1, anti-p-JNK, anti-JNK, anti-p-eIF2α, anti-eIF2α, anti-IRE1α, anti-CHOP, anti-NLRP3, anti-COX IV, anti-IL1β, anti-cleaved caspase1, anti-p-IκB, anti-IκB, anti-NFκB, and anti-cytochrome c antibodies were from Cell Signaling Technology; anti-ATF6 and anti-p-IRE1α antibodies were from Novus Biological; anti-p-ASK1(Ser967) antibody was from Sigma-Aldrich; anti-XBP1s antibody was from BioLegend; anti-TXNIP antibody was from MBL International Corporation; anti-WT-1 antibodies were from Santa Cruz Biotechnology or Abcam; and anti-ATF4 and anti-Trx2 antibodies were from Santa Cruz Biotechnology. HRP-conjugated anti-mouse β-actin antibody was from Sigma-Aldrich and HRP-conjugated secondary antibodies were from Cell Signaling Technology.

Park S.J., Kim Y., Li C., Suh J., Sivapackiam J., Goncalves T.M., Jarad G., Zhao G., Urano F., Sharma V, & Chen Y.M. (2022). Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome. Proceedings of the National Academy of Sciences of the United States of America, 119(35), e2116505119.

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