Proadifen hydrochloride

Chloroxylenol, metyrapone, proadifen hydrochloride, 1-aminobenzotriazole, sodium azide, 2,20-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and N, O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) were obtained from Sigma-Aldrich (Poznań, Poland). High purity grade ethyl acetate and 96% ethanol were purchased from POCH S.A. (Gliwice, Poland). All PCR reagents came from Applied Biosystems (Foster City, CA, USA). All the other chemicals were from Sigma-Aldrich (Darmstadt, Germany), POCH S.A. (Gliwice, Poland) and Chempur (Piekary Śląskie, Poland).

All the standards molecules (morphine 1 mg/mL in methanol and 6-MAM 1 mg/mL in acetonitrile) involved in this study were purchased from Sigma-Aldrich and stored at -20 °C. The Internal Standard (IS) SKF 525-A (Proadifen hydrochloride, analytical standard, > 95%, 100 mg) was purchased from Sigma-Aldrich as well.
Working solutions of each molecule and IS were prepared with methanol or acetonitrile at 100 µg/mL and 1 µg/mL, starting from standard solutions, and stored at -20 °C until use.
Solvents used in the extraction processes were purchased from Sigma-Aldrich (methanol) and from PanReac AppliChem ITW Reagents (phosphate buffer solution pH 4, phosphate buffer solution pH 9 and water for UV, HPLC, ACS).

Analytical standards involved in this study as Cocaine Multi-component Mixture solution (250 µg/mL in acetonitrile, certified reference material Cerilliant®), and Lidocaine solution (1 mg/mL in methanol, certified reference material Cerilliant®), were purchased from Sigma-Aldrich and stored at -20 °C. Internal Standard (IS) SKF 525-A (Proadifen hydrochloride, analytical standard, > 95%, 100 mg) was purchased from Sigma-Aldrich as well. Working solutions of the analytical standards and IS were prepared in acetonitrile or methanol and stored at -20 °C until use. Solvents used in the extraction processes were purchased by PanReac AppliChem ITW Reagents (methanol, phosphate buffer solution pH 9 and phosphate buffer pH 6, 2-propanol, n-hexane, n-heptane, and water).