RT was performed by using iScript Reverse Transcription Supermix kit (Bio-Rad). PCR reactions were run on a CFX96 Touch Real Time PCR (Bio-Rad) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad). Primers for qPCR (Cldn2, Dlx1, Dlx5, Etv5, Gabrb1, Gabrg2, Gabra3, Gad2, Gapdh, Itgb3, Lhx1, Lhx6, Mef2c, Nkx2.1, Nkx2.2, Nrp1, VegfA, Wnt10a, Wnt10b) were obtained from Thermo Fisher Scientific. The housekeeping gene Gapdh was used as a reference. The relative gene expression among different samples and subsequent fold increase in Vgatfl/fl versus VgatECKO endothelial cells or GABAergic neurons was determined according to published methodology44 (link).
Itgb3
Manufactured by Thermo Fisher Scientific
Itgb3 is a laboratory equipment product that serves as a core functional component. It is designed to facilitate research and analysis activities in various scientific domains. The precise details and intended uses of this product are not included in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Thermo Fisher Scientific (2 mentions)
Ssoadvanced universal sybr green supermix, by Bio-Rad (1 mentions)
Vegf a, by Thermo Fisher Scientific (1 mentions)
Cfx96 touch real time pcr, by Bio-Rad (1 mentions)
Iscript reverse transcription supermix kit, by Bio-Rad (1 mentions)
Cldn2, by Thermo Fisher Scientific (1 mentions)
Gabrb1, by Thermo Fisher Scientific (1 mentions)
Wnt10b, by Thermo Fisher Scientific (1 mentions)
Mef2c, by Thermo Fisher Scientific (1 mentions)
Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions)
Sybr green, by Roche (1 mentions)
Lightcycler, by Roche (1 mentions)
7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions)
Mirneasy mini kit, by Qiagen (1 mentions)
2 protocols using itgb3
1
Quantitative Analysis of Gene Expression
2
Quantitative Analysis of miRNA Expression in NSCLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Quantitative RT-PCR was performed using RNA isolated from NSCLC cells. Briefly, total RNA was extracted using an miRNeasy Mini Kit (217004, Qiagen), in accordance with the manufacturer’s instructions. cDNA was synthesized with a Taqman microRNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA). RT-PCR was performed on a LightCycler using SYBR Green (Roche, Basel, Switzerland) and a 7900HT Fast Real-Time PCR system (Applied Biosystems). Taqman gene expression assays (miR-150: hsa-miR-150-5p, A25576; miR-142: has-miR-142-5p, 4427975; ITGB3: Hs01001469_m1, 4453320; ITGAv: Hs00233808_m1, 4331182; GAPDH: Hs03929097_g1, 4453320) were purchased from Thermo Fisher Scientific. The comparative Ct method 2−ΔΔCt was used to calculate the changes in miRNA expression of all samples relative to a non-diseased sample, which was designated as the calibrator.
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