The subcellular localization of fluorescent fusion proteins was evaluated using a Nikon C1 confocal system built on a TE2000E platform and equipped with a 60× Plan-Apochromat oil immersion objective (Nikon Instruments B.V. Europe, Amsterdam, The Netherlands). The EGFP fusion proteins were excited with a Sapphire 488-nm laser (Coherent, Santa Clara, CA, USA) and observed using a 515/530-nm emission filter. Confocal images were processed and analyzed using EZ-C1 3.60 Nikon FreeViewer software.
Ez c1 3.60 freeviewer software
Manufactured by Nikon
The EZ-C1 3.60 Nikon FreeViewer software is a tool designed to view and analyze images captured using Nikon microscopes and digital cameras. It provides basic image viewing and processing capabilities.
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Lab products found in correlation
2 protocols using ez c1 3.60 freeviewer software
1
Confocal Imaging of Fluorescent Fusion Proteins
2
Confocal Microscopy of Fluorescent Proteins
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The subcellular localization of the fluorescent fusion proteins was evaluated using a Nikon C1 confocal system built on a TE2000E platform and equipped with a 60
Plan-Apochromat oil immersion objective (Nikon Instruments B.V. Europe, Amsterdam, The Netherlands). The fluorescence of the GFP/YFP fusion proteins was excited with a Sapphire 488 nm laser (Coherent, Santa Clara, CA, USA) and observed at 515/530 nm. The 543 nm line of a He-Ne laser (Melles Griot, NY, USA)
with a 650 nm long pass filter was used for chlorophyll detection. The confocal images were processed and analyzed using EZ-C1 3.60 Nikon FreeViewer software.
Plan-Apochromat oil immersion objective (Nikon Instruments B.V. Europe, Amsterdam, The Netherlands). The fluorescence of the GFP/YFP fusion proteins was excited with a Sapphire 488 nm laser (Coherent, Santa Clara, CA, USA) and observed at 515/530 nm. The 543 nm line of a He-Ne laser (Melles Griot, NY, USA)
with a 650 nm long pass filter was used for chlorophyll detection. The confocal images were processed and analyzed using EZ-C1 3.60 Nikon FreeViewer software.
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