Pure titanium substrates (purity 99.99+%) were purchased from Goodfellow Cambridge Ltd (Huntingdon, UK). Titanium coated quartz crystals (purity 99.995%, AT-cut, 5 MHz, 1 inch diameter) were purchased from Testbourne Ltd (Basingstoke, UK). Silicon nitride atomic force microscope (AFM) cantilevers were purchased from BudgetSensors (Sofia, Bulgaria). Fatty acid free (>99%) bovine serum albumin (BSA) was purchased from Sigma-Aldrich Company Ltd (Dorset, UK). Phosphate buffer saline (PBS) and sodium dodecyl sulphate (SDS) were purchased from Fisher Scientific (Loughborough, UK).
Sodium dodecyl sulphate (sds)
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, Germany
Sodium dodecyl sulphate (SDS) is a chemical compound commonly used in laboratory applications. It functions as an anionic surfactant, which helps solubilize and denature proteins for analysis and separation purposes. SDS is a key component in various biochemical techniques, such as gel electrophoresis and Western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Thermo Fisher Scientific (4 mentions)
Dipotassium hydrogen orthophosphate, by Thermo Fisher Scientific (4 mentions)
Disodium hydrogen orthophosphate, by Thermo Fisher Scientific (3 mentions)
Potassium dihydrogen orthophosphate, by Thermo Fisher Scientific (3 mentions)
D glucose, by Thermo Fisher Scientific (3 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions)
Ethyl acetate, by ITW Reagents (2 mentions)
Dinitrophenylhidrazine, by Thermo Fisher Scientific (2 mentions)
Guanidine hydrochloride for molecular biology, by Merck Group (2 mentions)
Potassium chloride (kcl), by Lach-Ner (2 mentions)
Sodium chloride (nacl), by Lach-Ner (2 mentions)
Mannitol, by Thermo Fisher Scientific (2 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
N heptane, by HiMedia (2 mentions)
Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions)
Pure titanium substrates, by Goodfellow (1 mentions)
Protein g magnetic beads, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Bioruptor, by Diagenode (1 mentions)
12 protocols using sodium dodecyl sulphate (sds)
1
Titanium Surface Characterization Protocol
2
Co-immunoprecipitation Assay Protocol
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Cells were lysed in Cell Signalling Lysis buffer complemented with Complete Mini Protein inhibitors (Roche), 5 mM dithiothreitol and 10% (v/v) glycerol, and sonicated using a Bioruptor (Diagenode). The co‐immunoprecipitations were carried out according to the manufacturer's protocol (Dynabeads® Protein G Immunoprecipitation Kit, Life Sciences Technology). Briefly, for each immunoprecipitation 8 μg antibody was conjugated to 50 µL Protein‐G magnetic beads (Millipore). After preclearing with normal mouse or rabbit IgG, 500 μg protein lysate was incubated overnight with the antibody‐coated beads at 4 °C with rotation. Beads were washed twice in IP lysis buffer and once in PBS and boiled in 1× Laemmli sample buffer (312.5 mM Tris–HCl pH 7.4, 50% (v/v) glycerol, 10% (w/v) SDS (Sodium Dodecyl Sulphate; Fisher Scientific), 5% (v/v) 2‐Mercaptoethanol (Sigma) before SDS‐PAGE.
3
Analytical Standards for Pharmaceutical Assays
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Furosemide (≥ 98% (HPLC), (±)-metoprolol (+)-tartrate salt (≥ 98% (titration)), 1,1dimethylbiguanide hydrochloride (metformin hydrochloride, 97%), danazol (≥ 98%), itraconazole (≥ 98% (TLC)), propafenone hydrochloride (≥ 98% (HPLC)), celecoxib (≥ 98% (HPLC)), azithromycin (≥ 95% (NT)) and atovaquone (≥ 98% (HPLC)) were all purchased from Sigma-Aldrich, UK. Nifedipine (98 to 102% (on dried substance)), paracetamol (97.5% min. (HPLC)), atorvastatin calcium (pharmaceutical secondary standard; traceable to USP, PhEur), atenolol (≥ 98% (TLC)) and ketoconazole (inclusive between 98%) were all purchased from Fisher Scientific, UK. Pravastatin sodium (≥ 98%) and lapatinib (≥ 99% (HPLC)) were purchased from Carbosynth, UK. MK-C1, MK-C2, MK-C3 and MK-C4 were provided by Merck & Co, INC, US. Sodium chloride, sodium hydroxide, sodium dodecyl sulphate, sodium acetate trihydrate, dipotassium hydrogen orthophosphate, potassium dihydrogen phosphate, ammonium acetate, hydrochloric acid 37% glacial acetic acid ≥ 99% and trichloroacetic acid 10% w/v were all purchased from Fisher Scientific, UK. HPLC grade methanol, ethanol, acetonitrile, acetone, trifluoroacetic acid (≥ 99%) were all purchased from Sigma-Aldrich, UK.
3.6% fat UHT milk was commercially purchased (Sainsbury's, UK).
3.6% fat UHT milk was commercially purchased (Sainsbury's, UK).
4
YEPD Media Preparation Protocol
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All Media chemicals YEPD (Yeast Extract Peptone Dextrose), Agar, Horse Serum, n- heptane, was purchased from Himedia (Mumbai, India). Methylene Blue (MB), Sodium Chloride (NaCl), Potassium Chloride (KCl), Mannitol, di-Sodium Hydrogen Orthophosphate, Potassium Di-hydrogen Orthophosphate, di-Potassium Hydrogen Orthophosphate, Sodium Hydroxide, D-Glucose, Sodium Dodecyl Sulphate (SDS) were obtained from Fischer Scientific.
5
Simultaneous Quantification of Triple-Drug Inhaler
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Analytical reagent grades for all the applied chemicals and HPLC grade for applied solvents were utilized throughout the analysis.
Formoterol fumarate (99.92% purity), tiotropium bromide (99.80% purity), and ciclesonide (99.40% purity) were friendly supplied by sigma pharmaceutical industries company in Steinheim, Germany. They were analyzed as delivered without further purification.
HPLC-grade methanol, acetonitrile, and sodium dodecyl sulphate were purchased from Fischer chemicals in Germany. Orthophosphoric acid was purchased from El-Nasr pharmaceutical company in Egypt.
Triohale Inhaler; (Label claim: 6.0 μg Formoterol fumarate, 9.0 μg tiotropium bromide, and 200.0 μg ciclesonide /buff) (delivered dose), manufactured by Cipla was obtained from commercial sources.
Formoterol fumarate (99.92% purity), tiotropium bromide (99.80% purity), and ciclesonide (99.40% purity) were friendly supplied by sigma pharmaceutical industries company in Steinheim, Germany. They were analyzed as delivered without further purification.
HPLC-grade methanol, acetonitrile, and sodium dodecyl sulphate were purchased from Fischer chemicals in Germany. Orthophosphoric acid was purchased from El-Nasr pharmaceutical company in Egypt.
Triohale Inhaler; (Label claim: 6.0 μg Formoterol fumarate, 9.0 μg tiotropium bromide, and 200.0 μg ciclesonide /buff) (delivered dose), manufactured by Cipla was obtained from commercial sources.
6
Trout Feed Protein Composition Analysis
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NaCl, KCl and trichlorоacetic acid (TCA) were purchased from Lachner (Neratowitz, Czech Republic). Sodium dodecyl sulphate was purchased from Thermo Fisher (Kandel, Germany). Dinitrophenylhidrazine was purchased from Acros Organics (Geel, Belgium). Ethyl acetate was obtained from Panreac (Barcelona, Spain). Guanidine hydrochloride for molecular biology was purchased from Sigma Aldrich (St. Louis, USA) and ethanol was obtained from Reahem (Srbobran, Serbia). Extruded rainbow trout feed was purchased from a local fish feed market and used in this research. The particular fish feed manufacturer was chosen due to the various protein sources, that the rainbow trout feed contained. Fish meal, soy concentrate, wheat meal, haemoglobin, corn and gluten were the protein sources in the extruded rainbow trout feed.
7
Trout Feed Protein Composition Analysis
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NaCl, KCl and trichlorоacetic acid (TCA) were purchased from Lachner (Neratowitz, Czech Republic). Sodium dodecyl sulphate was purchased from Thermo Fisher (Kandel, Germany). Dinitrophenylhidrazine was purchased from Acros Organics (Geel, Belgium). Ethyl acetate was obtained from Panreac (Barcelona, Spain). Guanidine hydrochloride for molecular biology was purchased from Sigma Aldrich (St. Louis, USA) and ethanol was obtained from Reahem (Srbobran, Serbia). Extruded rainbow trout feed was purchased from a local fish feed market and used in this research. The particular fish feed manufacturer was chosen due to the various protein sources, that the rainbow trout feed contained. Fish meal, soy concentrate, wheat meal, haemoglobin, corn and gluten were the protein sources in the extruded rainbow trout feed.
8
Fungal Cell Wall Characterization Protocol
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All media chemicals YEPD (yeast extract peptone dextrose), agar, rhodamine 6G (R6G), 2-deoxy-D-glucose (2-DOG), 2,4-dinitrophenol (2,4 DNP), and n-heptane, was purchased from HiMedia (Mumbai, India). Sodium chloride (NaCl), calcium chloride (CaCl2), lithium chloride (LiCl), potassium chloride (KCl), mannitol, disodium hydrogen orthophosphate, potassium dihydrogen orthophosphate, dipotassium hydrogen orthophosphate, sodium hydroxide, D-glucose, and sodium dodecyl sulphate (SDS) were obtained from Fischer Scientific. Calcofluor white (CFW), Congo red (CR), and sesamol (Ses) were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
9
Comprehensive Microbial Culture Media Protocols
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All Media chemicals YEPD (Yeast Extract Peptone Dextrose), nutrient broth, yeast nitrogen base w/o amino acid and ammonium sulphate (YNB w/o amino acid and ammonium sulphate), agar, rhodamine 6G (R6G), 2-deoxy glucose (2-DOG), horse serum, 2,4 dinitrophenol (2,4 DNP), n- heptane, formamide, osmium tetroxide (OsO4), hexamethyldisilizane (HMDS), glutaraldehyde, propidium iodide were purchased from Himedia (Mumbai, India). Sodium chloride (NaCl), calcium chloride (CaCl2), lithium chloride (LiCl), potassium chloride (KCl), mannitol, di-sodium hydrogen orthophosphate, potassium di-hydrogen orthophosphate, di-potassium hydrogen orthophosphate, sodium hydroxide, D-glucose, sodium dodecyl sulphate (SDS), potassium hydroxide (KOH), ammonium sulphate ((NH4)2SO4), dimethyl sulphoxide (DMSO) were obtained from Fischer Scientific. Tetrazolium salt 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) was purchased from SRL, Mumbai. Calcofluor white (CFW), congo red (CR), perillyl alcohol (PA), diethyl pyrocarbonate (DEPC), 4-morpholinepropanesulfonic acid (MOPS), tri-reagent, DNase were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
10
In Vitro Dissolution Testing: Simulated Gastrointestinal Media Preparation
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ALG, BSA lyophilized powder, sodium bicarbonate, 2,4,6-trinitrobenzenesulfonic acid (TNBS), CaCl2 and 4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES), CIP and IND were all purchased from Sigma-Aldrich, Dorset, UK. Sodium dodecyl sulphate was purchased from ACROS, Leicestershire, UK.
The British Pharmacopeia standard was used to prepare the simulated gastric medium (SGM) and simulated intestinal medium (SIM) as follows:
The British Pharmacopeia standard was used to prepare the simulated gastric medium (SGM) and simulated intestinal medium (SIM) as follows:
SGM: 0.1M hydrochloric acid (pH 1.2).
SIM: 2.4g HEPES and 8.5g NaCl were dissolved in 1 L distilled water to obtain concentrations of 9.2 mM and 150 mM respectively. The pH was then adjusted to 6.5 using diluted HCl or NaOH solutions as required.
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