The monkey kidney COS-7 cells were purchased from ATCC (Manassas, VA). [3H]-labeled estrone sulfate ([3H]-ES) was purchased from PerkinElmer (Waltham, MA). Membrane-impermeable biotinylation reagent NHS-SS-biotin, streptavidin-agarose beads and protein G-agarose beads were purchased from Pierce (Rockford, IL). cDNA for human Nedd4-2 was generously provided by Dr. Peter M. Snyder of the College of Medicine, University of Iowa (Iowa City, IA). Mouse anti-myc antibody (9E10) was purchased from Roche (Indianapolis, IN). Rabbit anti-Nedd4-2 and mouse anti-E-cadherin antibodies were purchased from Abcam (Cambridge, MA). Mouse anti-flag antibody was purchased from Sigma–Aldrich (St. Louis, MO). Mouse anti-ubiquitin, mouse anti-β-actin, and mouse anti-phospho-tyrosine (anti-p-tyr) antibodies were purchased from Santa Cruz (Santa Cruz, CA). Nedd4-2 siRNA oligonucleotides (Silencer® Select, identification number S23570) were purchased from Ambion (Grand Island, NY). AG490 and all other reagents were purchased from Sigma–Aldrich (St. Louis, MO).
Mouse anti phospho tyrosine anti p tyr antibodies
Manufactured by Santa Cruz Biotechnology
Mouse anti-phospho-tyrosine (anti-p-tyr) antibodies are a laboratory reagent used in Western blotting, immunoprecipitation, and other techniques to detect and quantify proteins with phosphorylated tyrosine residues. These antibodies specifically recognize and bind to tyrosine-phosphorylated proteins, enabling researchers to study signal transduction pathways and protein-protein interactions.
Automatically generated - may contain errors
Lab products found in correlation
Cos 7 cells, by American Type Culture Collection (2 mentions)
Mouse anti e cadherin antibody, by Abcam (2 mentions)
Silencer select, by Thermo Fisher Scientific (2 mentions)
Mouse anti ubiquitin, by Santa Cruz Biotechnology (2 mentions)
Ag490, by Merck Group (2 mentions)
Mouse anti β actin, by Santa Cruz Biotechnology (2 mentions)
Mouse anti flag antibody, by Merck Group (2 mentions)
Mouse anti myc antibody 9e10, by Roche (2 mentions)
3h labeled estrone sulfate 3h es, by PerkinElmer (2 mentions)
Rabbit anti nedd4 2, by Abcam (2 mentions)
2 protocols using mouse anti phospho tyrosine anti p tyr antibodies
1
Monkey Kidney Cells Membrane Protein Internalization
2
Monkey Kidney Cells Membrane Protein Internalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The monkey kidney COS-7 cells were purchased from ATCC (Manassas, VA). [3H]-labeled estrone sulfate ([3H]-ES) was purchased from PerkinElmer (Waltham, MA). Membrane-impermeable biotinylation reagent NHS-SS-biotin, streptavidin-agarose beads and protein G-agarose beads were purchased from Pierce (Rockford, IL). cDNA for human Nedd4-2 was generously provided by Dr. Peter M. Snyder of the College of Medicine, University of Iowa (Iowa City, IA). Mouse anti-myc antibody (9E10) was purchased from Roche (Indianapolis, IN). Rabbit anti-Nedd4-2 and mouse anti-E-cadherin antibodies were purchased from Abcam (Cambridge, MA). Mouse anti-flag antibody was purchased from Sigma–Aldrich (St. Louis, MO). Mouse anti-ubiquitin, mouse anti-β-actin, and mouse anti-phospho-tyrosine (anti-p-tyr) antibodies were purchased from Santa Cruz (Santa Cruz, CA). Nedd4-2 siRNA oligonucleotides (Silencer® Select, identification number S23570) were purchased from Ambion (Grand Island, NY). AG490 and all other reagents were purchased from Sigma–Aldrich (St. Louis, MO).
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