The transcription patterns of GhWAKs in cotton roots after inoculation with V. dahliae were analyzed using high-through RNA-seq data published previously [37 (link)]. Log2Fold change were calculated from FPKM (fragments per kilobase of exon model per million mapped) and used for the heat map of hierarchical clustering with the TBtools v0.67 software [42 ]. Total RNA was extracted using EASYspin Plant RNA kit (Aidlab, Beijing, China) according to the manufacturer’s instructions. The quality and concentration of RNA were detected by 1.5% agarose gel electrophoresis and NanoDrop™ 1000 spectrophotometer (Thermo Fisher Scientific), respectively. cDNA was synthesized with a reverse transcription kit (ReverTra Ace® qPCR RT Master Mix with gDNA Remover, TaKaRa, Dalian, China). qRT-PCR was performed using 7500 Real Time PCR System (Applied Biosystems, USA) with THUNDERBIRD®SYBR® qPCR Mix (TaKaRa, Dalian, China). The 2-ΔΔCt method was used to calculate the relative expression of genes. GhHis 3 was used as internal reference. Three biological repeats were taken for each treatment.
Revertra ace qpcr rt master mix with gdna remover
Manufactured by Takara Bio
Sourced in China
ReverTra Ace® qPCR RT Master Mix with gDNA Remover is a reagent kit designed for reverse transcription and real-time PCR. It includes components for cDNA synthesis and removal of genomic DNA contamination.
Automatically generated - may contain errors
2 protocols using revertra ace qpcr rt master mix with gdna remover
1
Transcriptional Patterns of GhWAKs in Cotton Roots
2
Transcriptional Profiling of GhWAKs in Cotton Roots under Verticillium Wilt
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The transcription patterns of GhWAKs in cotton roots after inoculation with V. dahliae were analyzed using high-through RNA-seq data published previously [37] . Log 2 Fold change were calculated from FPKM (fragments per kilobase of exon model per million mapped) and used for the heat map of hierarchical clustering with the TBtools v0.67 software [42] . Total RNA was extracted using EASYspin Plant RNA kit (Aidlab, Beijing, China) according to the manufacturer's instructions. The quality and concentration of RNA were detected by 1.5% agarose gel electrophoresis and NanoDrop TM 1000 spectrophotometer (Thermo Fisher Scienti c), respectively. cDNA was synthesized with a reverse transcription kit (ReverTra Ace ® qPCR RT Master Mix with gDNA Remover, TaKaRa, Dalian, China). qPCR was performed using 7500
Real Time PCR System (Applied Biosystems, USA) with THUNDERBIRD ® SYBR ® qPCR Mix (TaKaRa, Dalian, China). The 2 -ΔΔCt method was used to calculate the relative expression of genes. GhHis 3 was used as internal reference. Three biological repeats were taken for each treatment.
Real Time PCR System (Applied Biosystems, USA) with THUNDERBIRD ® SYBR ® qPCR Mix (TaKaRa, Dalian, China). The 2 -ΔΔCt method was used to calculate the relative expression of genes. GhHis 3 was used as internal reference. Three biological repeats were taken for each treatment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!