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Cgp 35348 hydrate

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CGP 35348 hydrate is a chemical compound used in laboratory settings. It functions as a research tool for studying certain biological processes. The product details and intended uses are factual and do not include any interpretation or extrapolation beyond its core function.

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Lab products found in correlation

Spss 21, by IBM (2 mentions) Bicuculline methiodide, by Merck Group (2 mentions) Indomethacin, by Merck Group (1 mentions) Bicuculline, by Merck Group (1 mentions) Tiagabine, by Merck Group (1 mentions)

3 protocols using cgp 35348 hydrate

1

Antinociceptive Effects of Enaminones and GABAergic Drugs

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Indomethacin (Sigma-Aldrich, St Louis, MO, USA) was dissolved in phosphate buffered saline (PBS); tiagabine, bicuculline and CGP 35348 hydrate (Sigma-Aldrich, St Louis, MO, USA) in normal saline (NaCl 0.9%) and enaminones (resynthesized in-house6 (link)9 (link)) in peanut oil. The drugs were freshly prepared before administration and administered intraperitoneally (i.p.) to mice at a volume of 10 ml/kg body mass. Indomethacin and tiagabine were administered to mice 1 hour before subcutaneous (s.c.) administration of formalin (5%; 20 μl). Enaminones were administered 1.5 hours before administration of formalin, taking into consideration the time the enaminone E139 produced significant antinociceptive effect in the hot plate test15 . bicuculline, a GABAA receptor antagonist, and CGP 35348 hydrate, a GABAB receptor antagonist, were administered 15 minutes before the administration of E139 for the hot plate test.
Masocha W., Kombian S.B, & Edafiogho I.O. (2016). Evaluation of the antinociceptive activities of enaminone compounds on the formalin and hot plate tests in mice. Scientific Reports, 6, 21582.
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2

Intrathecal Pharmacological Manipulation

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Bicuculline methiodide (0.3µg intrathecally) was purchased from Sigma, dissolved in saline and 10 µl were injected89 (link), 90 (link). CGP 35348 hydrate (30µg) was purchased from Sigma, dissolved in saline and 10 µl was injected intrathecally. To compare the difference between the time-course curves of the behavioral testing, we used two-way ANOVA with one within-subjects factor (time) and one between-subjects factor (group) of a general linear model with repeated-measures analysis with IBM SPSS 21 (IBM, Armonk, NY, USA). The statistical significance of the differences of neurochemical changes was determined by the two-tailed t-test or one-way ANOVA post hoc test following Fisher’s protected least significant difference (StatView, Cary, NC). All data were presented as mean±SEM. and P-values of < 0.05 were considered to be statistically significant. The sample size estimate was based on our previous studies15 , 54 (link). For the detailed numbers of animals in each group, see Table 1.
Kanda H., Kanao M., Liu S., Yi H., Iida T., Levitt R.C., Candiotti K.A., Lubarsky D.A, & Hao S. (2016). HSV vector-mediated GAD67 suppresses neuropathic pain induced by perineural HIV gp120 in rats through inhibition of ROS and Wnt5a. Gene therapy, 23(4), 340-348.
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3

Intrathecal Pharmacological Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bicuculline methiodide (0.3µg intrathecally) was purchased from Sigma, dissolved in saline and 10 µl were injected89 (link), 90 (link). CGP 35348 hydrate (30µg) was purchased from Sigma, dissolved in saline and 10 µl was injected intrathecally. To compare the difference between the time-course curves of the behavioral testing, we used two-way ANOVA with one within-subjects factor (time) and one between-subjects factor (group) of a general linear model with repeated-measures analysis with IBM SPSS 21 (IBM, Armonk, NY, USA). The statistical significance of the differences of neurochemical changes was determined by the two-tailed t-test or one-way ANOVA post hoc test following Fisher’s protected least significant difference (StatView, Cary, NC). All data were presented as mean±SEM. and P-values of < 0.05 were considered to be statistically significant. The sample size estimate was based on our previous studies15 , 54 (link). For the detailed numbers of animals in each group, see Table 1.
Kanda H., Kanao M., Liu S., Yi H., Iida T., Levitt R.C., Candiotti K.A., Lubarsky D.A, & Hao S. (2016). HSV vector-mediated GAD67 suppresses neuropathic pain induced by perineural HIV gp120 in rats through inhibition of ROS and Wnt5a. Gene therapy, 23(4), 340-348.
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