Cells and exosomes were lysed by RIPA lysis buffer, and the total protein was extracted. The denatured protein sample (20 μg) was separated by 12% SDS-PAGE and transferred to the polyvinylidene fluoride membrane. The membrane was blocked with 5% skimmed milk and probed with primary antibodies (Abcam) against CD133 (1:1000, ab19898), Nestin (Mouse, 1:2000, ab254048), GFAP (1:10,000, ab7260), Oct4 (1:1000, ab181557), Sox2 (1:1000, ab92494), MLL4 (Mouse, 1:1000, ab56770), H3K4me1 (1:5000, ab176877), H3K9me3 (1:1000, ab8898) and β-actin (1:5000, ab179467). The peroxidase-labeled goat anti-rabbit or anti-mouse IgG (H+L) served as secondary antibody. The blots were developed by chemiluminescence system (Millipore, Billerica, MA). The density of western blot band was quantified by ImageJ software, and normalized to β-actin.
Ab56770
Manufactured by Abcam
Sourced in United Kingdom
Ab56770 is a lab equipment product manufactured by Abcam. It is a device designed for specific laboratory applications. The core function of this product is to facilitate certain experimental or analytical processes within a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ab92494, by Abcam (1 mentions)
Ab19898, by Abcam (1 mentions)
Ab181557, by Abcam (1 mentions)
Ab8898, by Abcam (1 mentions)
Chemiluminescence system, by Merck Group (1 mentions)
Ab7260, by Abcam (1 mentions)
Ab176877, by Abcam (1 mentions)
Ab254048, by Abcam (1 mentions)
Ab179467, by Abcam (1 mentions)
2 protocols using ab56770
1
Comprehensive Protein Analysis of Cells and Exosomes
2
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole cell lysates from the cell lines were prepared by lysing the cells in ice-cold RIPA buffer. Total cell lysates were prepared and 20 µg of proteins was subjected to Western blot analysis in 12% SDS-PAGE gels electrophoresis under denaturing and reducing conditions. Specific primary antibodies for SEMA3B (rabbit anti-human polyclonal antibody, ab48197; Abcam, UK), MLL4 (mouse anti-human monoclonal antibody, ab56770; Abcam, UK), and ZNF143 (rabbit anti-human polyclonal antibody, ab96650; Abcam, UK) were, respectively, used. GAPDH was used as a loading control.
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