Hrp anti hemagglutinin 12013819001

The TRIM45 expression plasmid was constructed using the GATEWAY System (Life Technologies, Carlsbad, CA, USA). The HA-tagged p53 expression plasmid and the p53 domain deletion mutants were kindly provided by Dr. Tiebang Kang (Sun Yat-sen University, Guangzhou, China). All mutants were generated using standard molecular techniques. The anti-TRIM45 antibody (NBP1-53109) was purchased from Novus (Littleton, CO, USA) and anti-p53 (sc-126) was obtained from Santa Cruz (Santa Cruz, CA, USA). Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592) and anti-β-actin (A1978) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) and anti-c-Myc-HRP (11814150001) were purchased from Roche Applied Science (Mannheim, Germany). The anti-caspase-3 (25546-1-AP) was purchased from Proteintech (Wuhan, Hubei, P.R.C). The anti-MDM2 (YT5186) was purchased from ImmunoWay (Newark, DE, USA). The anti-K63-linked ubiquitin antibody (05-1308) was obtained from Millipore (Billerica, MA, USA). The anti-phospho-Akt (no. 4060), anti-Akt (no. 4691), anti-phospho-JNK (no. 9251), anti-JNK (no. 9252), anti-phospho-ERK (no. 9101), anti-ERK (no. 9102), anti-phospho-p38 (no. 9211), anti-p38 (no. 9212), anti-Puma (no. 4976), and anti-K48-linked ubiquitin (no. 4289) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

Anti-β-actin (A1978), horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-mouse-IgG-HRP (AP308P), and anti-rabbit-IgG-HRP (AP132P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). Anti-c-Myc (HT101) was purchased from TransGen Biotech (Beijing, China). Anti-TAK1 (5206) and p65 (8242) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). Anti-CHIP (sc-133066) was purchased from Santa Cruz Biotechnology (San Diego, CA, USA). TNFα Recombinant Human Protein (10602HNAE25) was purchased from Thermo Fisher (Waltham, MA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from SHSY5Y cDNA and then subcloned into the pcDNA3.1 vector. The recombinant plasmids were confirmed by DNA sequencing in Sangon Biotech (Shanghai, China).

Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592) and anti-β-actin (A1978) were purchased from Sigma; HRP-anti-hemagglutinin (12013819001), anti-c-Myc-HRP (11814150001) were purchased from Roche Applied Science; anti-phospho-IKKα/β (No.2697S), anti-phospho-JNK (No.9251), anti-JNK (No.9252), anti-phospho-ERK (No.9101), anti-ERK (No.9102), anti-phospho-p38 (No.9211), anti-p38 (No.9212), anti-p65 (No.6956), anti-IκBα (No.4814) and anti-phospho-IkBα (Ser32/36) (No. 9246) were from Cell Signaling Technology; anti-NEMO (sc-8300), anti-USP18(sc-98431) were purchased from Santa Cruz Biotechnology; anti-IKKβ were purchased from Millipore; CF568 Goat anti-mouse IgG (H+L) (20101-1) were purchased from Biotium; anti-linear polyubiquitin-specific monoclonal antibody (AB130) were purchased from LifeSensors.
Recombinant human TNF-α was purchased from PeproTech; Lipopolysaccharides (LPS) (L4391-1 MG) were purchased from Sigma. Pam3CSK4 and CL097 were purchased from InvivoGen.