Biotin-labeled miR-2861 was purchased from Genewiz (Jiangsu, China). Biotin-labeled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C overnight. Then, streptavidin-coated beads were incubated with SV40-MES-13 cell lysate at 4 °C for 12 h. RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan) and assessed through qRT-PCR.
Biotin labeled mir 2861
Manufactured by Genewiz
Sourced in China
Biotin-labeled miR-2861 is a synthetic, single-stranded RNA molecule labeled with biotin. It is designed to mimic the sequence and function of the naturally occurring microRNA, miR-2861. The biotin label allows for the detection and tracking of the miRNA in various experimental and analytical applications.
Automatically generated - may contain errors
2 protocols using biotin labeled mir 2861
1
Biotin-Labeled miR-2861 Pulldown Assay
2
Biotin-miR-2861 Affinity Purification
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Biotin-labeled miR-2861 was purchased from Genewiz (Jiangsu, China).
Biotin-labelled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermofisher Scientific) at 4 °C overnight. Then, streptavidin-coated beads were incubated with mesangial cell lysate at 4 °C for 12 h. RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan), and detected through qRT-PCR assay.
Biotin-labelled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermofisher Scientific) at 4 °C overnight. Then, streptavidin-coated beads were incubated with mesangial cell lysate at 4 °C for 12 h. RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan), and detected through qRT-PCR assay.
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