Mat incos 50

Melting points were measured in open capillary tubes on a BÜCHI B-545 melting point apparatus (BÜCHI Labortechnik AG, Flawil, Switzerland) and are uncorrected. The elemental analyses (C, H, N) were performed using the Perkin–Elmer 2400 CHN analyzer (PerkinElmer, Waltham, MA, USA) and were within ±0.4% of the theoretical values. The 500 MHz-¹H and 126 MHz-¹³C spectra were recorded on Bruker AVANCE-500 spectrometer and 2D spectra were recorded on a Bruker AVANCE-600 spectrometer (Bruker, Bremen, Germany). All spectra were recorded at room temperature except where indicated otherwise and were referenced internally to solvent reference frequencies. Chemical shifts (δ) are quoted in ppm and coupling constants (J) are reported in Hz. LC-MS spectra were obtained on a Finnigan MAT INCOS-50 (Thermo Finnigan LLC, San Jose, CA, USA). Solvents and reagents that are commercially available were used without further purification. The synthetic procedure for compound 1 was described in [48 (link)], for derivatives 2a–l in [29 (link)] and for compound 4 in [35 (link)].

The melting points were determined on a Kofler hot-stage apparatus and were uncorrected. ¹H and ¹³C NMR spectra were recorded on a Bruker AM-300 instrument (Bruker Ltd., Moscow, Russia) with TMS as the standard. J values are given in Hz. MS spectra (EI, 70 eV) were obtained with a Finnigan MAT INCOS 50 instrument (Thermo Finnigan LLC, San Jose, CA, USA). High-resolution MS spectra were measured on a Bruker micrOTOF II instrument using electrospray ionization (ESI). IR spectra were measured with a Bruker “Alpha-T” instrument (Bruker, Billerica, MA, USA) in KBr pellets. UV-vis spectra in the region 200–900 nm were registered for DMF solutions of 1–3 C= 10^–4 M in the standard 10 mm quartz cell using a Carl Zeiss Specord M400 spectrophotometer.