The following reagents were purchased for this study: Matrine from Shanghai Aladdin Company, MTT solution from Amresco (USA), ERK1/2 monoclonal antibody from Cell Signaling Technology, polymerase chain reaction (PCR) kit from Takara, western blot kit from Abcam, transwell (pore size 0.8 μm) from Corning. The HepG2 cell line was purchased from the Shanghai Institute of Biology at the Chinese Academy of Sciences.
Polymerase chain reaction kit
Manufactured by Takara Bio
Sourced in United States, Japan
The Polymerase Chain Reaction (PCR) kit is a laboratory equipment used for the amplification of DNA sequences. It facilitates the exponential replication of targeted DNA fragments, allowing for the generation of multiple copies from a small initial sample.
Automatically generated - may contain errors
Lab products found in correlation
Mtt solution, by Avantor (1 mentions)
Erk1 2 monoclonal antibody, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
5 aza cdr, by Merck Group (1 mentions)
Hindiii, by Takara Bio (1 mentions)
T4 ligase, by Takara Bio (1 mentions)
Xbai, by Takara Bio (1 mentions)
3 protocols using polymerase chain reaction kit
1
Matrine Inhibits HepG2 Cell Migration
2
Modulation of RhoC/ROCK pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RPMI-8226 and U266 cell lines were obtained from the cell bank of Center Laboratory, the Affiliated Hospital of Qingdao University; 5-Aza-CdR and TSA were purchased from Sigma-Aldrich (St. Louis, MO, USA); Roswell Park Memorial Institute 1640 (RPMI 1640) culture medium and fetal bovine serum (FBS) were purchased from Gibco (Rockville, MD, USA); Cell Counting Kit-8 (CCK-8) was purchased from Yiyuan Biotechnologies (Yiyuan, China); Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis double staining kit was purchased from Becton Dickinson (Franklin Lakes, NJ, USA); TRIzol, reverse transcription kit, and polymerase chain reaction (PCR) kit were purchased from TaKaRa (Tokyo, Japan); RhoC antibody was purchased from Cell Signaling Technology (Danvers, MA, USA), ROCK1 and ROCK2 antibody were purchased from Abcam (Cambridge, MA, USA); RhoC, ROCK1/ROCK2 primers and internal reference were synthesized by Shanghai Shenggong Bioengineering (Shanghai, China).
3
Plasmid Construction for Protein A and Uricase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The plasmid containing the gene of staphylococcal protein A (spa) and uricase (UOX), pET-28a-sUOX (accession no. D32043.1) (33 (link)–36 (link)), was preserved by the Department of Biochemistry and Molecular Biology, Chongqing Medical University, and the plasmid was purchased from Beijing Beina Chuanglian Biotechnology Research Institute. The pMG36e vector was purchased from the Suzhou Bei Na Chuanglian Biological Corporation. The pUC57 vector containing the S-layer protein signal peptide gene (spo) (accession no. Z14250.1) of Lactobacillus brevis was purchased from the Chengdu Qingke Zixi Biological Company. In this study, Escherichia coli DH5α and BL-21 cells were used for cloning and expression, respectively, which were grown in LB medium (Beijing Dingguo Changsheng Biotechnology Co., Ltd.) broth containing 200 μg/ml erythromycin incubated at 37°C with 200 r/min rotation. Restriction enzymes XbaI, HindIII, SalI and T4 ligase and polymerase chain reaction (PCR) kit (catalog no. R060A) were purchased from Takara Biotechnology Co., Ltd.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!