Intra-orbital unilateral ONC was performed as previously described (De Groef et al., 2016; (link)Geeraerts et al., 2016) (link). Briefly, the optic nerve was exposed via an incision in the conjunctiva and crushed 1 mm from the globe with a Dumont #7 cross-action forceps (Fine Science Tools) for 5 seconds. Fundoscopy was performed before and after the surgery to verify retinal perfusion and to exclude animals with ischemia. Eyes from naive animals were used as controls.
Dumont 7 cross action forceps
Manufactured by Fine Science Tools
Sourced in Germany
The Dumont #7 cross-action forceps are precision instruments designed for delicate handling and manipulation tasks in laboratory environments. These forceps feature a cross-action design, allowing for precise and controlled movements. The forceps are made from high-quality materials for durability and reliability.
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Lab products found in correlation
2 protocols using dumont 7 cross action forceps
1
Optic Nerve Crush Injury Procedure
2
Optic Nerve Crush Injury Protocol
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Unilateral ONC was performed as previously described52 (link). Briefly, the optic nerve was exposed via an incision in the conjunctiva and crushed 1 mm from the globe with a Dumont #7 cross-action forceps (Fine Science Tools, Germany) for 5 s. Animals were anesthetised via an intraperitoneal injection of ketamine (75 mg/kg body weight, Nimatek, Eurovet) and medetomidine (1 mg/kg, Domitor, Pfizer), which was reversed after the surgical procedure by atipamezole (1 mg/kg, Antisedan, Pfizer). In addition, local analgesia (oxybuprocaïne 0.4%, Unicaïne, Théa) was applied on the eye before the surgery, and antibiotic ointment (tobramycin 0.3%, Tobrex, Alcon) was applied afterwards. Animals were euthanized 7 days post crush.
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