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Bond edta solution

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Bond™EDTA solution is a laboratory reagent designed for use in various analytical techniques. It is a buffered solution containing EDTA (Ethylenediaminetetraacetic acid), a chelating agent. The primary function of this solution is to complex metal ions, which can be useful in sample preparation and analytical procedures.

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Lab products found in correlation

Bond citrate solution, by Leica camera (2 mentions) Bond proteolytic enzyme kit, by Leica camera (2 mentions) Bond primary antibody diluent, by Leica Biosystems (2 mentions) Scn400 slide scanner, by Leica Biosystems (2 mentions) Bond max, by Leica Biosystems (2 mentions) Bond polymer refine detection kit, by Leica Biosystems (2 mentions)

2 protocols using bond edta solution

1

Immunohistochemical Analysis of Liver Sections

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Livers were fixed overnight in 4% PFA prior to embedding and sectioning. Paraffin-embedded liver sections (2 μm) were processed for immunohistochemistry (IHC) thereafter. Automated staining was performed on BOND-MAX (Leica Biosystems). Antigen retrieval was carried out with Bond™citrate solution (AR9961, Leica), Bond™EDTA solution (AR9640, Leica) or Bond™ proteolytic enzyme kit (AR9551, Leica) after which sections were incubated with antibodies against antigens in Bond™ primary antibody diluent (AR9352, Leica Biosystems). Primary antibody exposure was followed by secondary antibody (Leica Biosystems) and staining using the Bond Polymer Refine Detection Kit (DS9800, Leica Biosystems). For quantification of stainings, slides were scanned using a SCN400 slide scanner (Leica Biosystems) at 20x magnification.
Guerrero A., Herranz N., Sun B., Wagner V., Gallage S., Guiho R., Wolter K., Pombo J., Irvine E.E., Innes A.J., Birch J., Glegola J., Manshaei S., Heide D., Dharmalingam G., Harbig J., Olona A., Behmoaras J., Dauch D., Uren A.G., Zender L., Vernia S., Martínez-Barbera J.P., Heikenwalder M., Withers D.J, & Gil J. (2019). Cardiac glycosides are broad-spectrum senolytics. Nature metabolism, 1(11), 1074-1088.
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2

Immunohistochemical Analysis of Liver Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Livers were fixed overnight in 4% PFA prior to embedding and sectioning. Paraffin-embedded liver sections (2 μm) were processed for immunohistochemistry (IHC) thereafter. Automated staining was performed on BOND-MAX (Leica Biosystems). Antigen retrieval was carried out with Bond™citrate solution (AR9961, Leica), Bond™EDTA solution (AR9640, Leica) or Bond™ proteolytic enzyme kit (AR9551, Leica) after which sections were incubated with antibodies against antigens in Bond™ primary antibody diluent (AR9352, Leica Biosystems). Primary antibody exposure was followed by secondary antibody (Leica Biosystems) and staining using the Bond Polymer Refine Detection Kit (DS9800, Leica Biosystems). For quantification of stainings, slides were scanned using a SCN400 slide scanner (Leica Biosystems) at 20x magnification.
Guerrero A., Herranz N., Sun B., Wagner V., Gallage S., Guiho R., Wolter K., Pombo J., Irvine E.E., Innes A.J., Birch J., Glegola J., Manshaei S., Heide D., Dharmalingam G., Harbig J., Olona A., Behmoaras J., Dauch D., Uren A.G., Zender L., Vernia S., Martínez-Barbera J.P., Heikenwalder M., Withers D.J, & Gil J. (2019). Cardiac glycosides are broad-spectrum senolytics. Nature metabolism, 1(11), 1074-1088.
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