The MDA-MB-231, MDA-MB-453 and MDA-MB-468 human BC cell lines were incubated in L15 medium (LA9510; Beijing Solarbio Science & Technology Co., Ltd.) containing 10% FBS (SH30084.03; HyClone; Cytiva), 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO2 incubator. The MCF-7 and ZR-75-1 cells were cultured with Minimum Essential Medium (MEM; 41500; Beijing Solarbio Science & Technology Co., Ltd.) containing 10% FBS, 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) and RPMI-1640 (31800; Beijing Solarbio Science & Technology Co., Ltd.) medium containing 10% FBS, 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO2 incubator, respectively. The MCF-10A cells were cultured in Mammary Epithelium Basal Medium (CC-3150; iCell Bioscience, Inc.) in a 37°C, 5% CO2 incubator. The MDA-MB-231, MDA-MB-453, MDA-MB-468, MCF-7 and MCF-10A cells were purchased from iCell Bioscience, Inc. The ZR-75-1 cells were purchased from Procell Life Science & Technology Co., Ltd.
S8290
Manufactured by Solarbio
Sourced in China
The S8290 is a laboratory centrifuge designed for general-purpose applications. It provides stable and reliable performance for a variety of sample processing needs.
Automatically generated - may contain errors
Lab products found in correlation
N8090, by Solarbio (1 mentions)
C8251, by Solarbio (1 mentions)
Mda mb 231, by Solarbio (1 mentions)
Mda mb 468, by Solarbio (1 mentions)
Hyclone, by Cytiva (1 mentions)
Mda mb 231, by iCell Bioscience (1 mentions)
Mda mb 468, by iCell Bioscience (1 mentions)
Mcf 7, by iCell Bioscience (1 mentions)
Mcf 10a, by iCell Bioscience (1 mentions)
2 protocols using s8290
1
Culturing Breast Cancer Cell Lines
2
Gut Microbiome Restoration in Piglets
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ampicillin (1 mg/ml) (A8180, Solarbio, China), streptomycin (1 mg/ml) (S8290, Solarbio, China), vancomycin (0.25 mg/ml) (V8050, Solarbio, China), and neomycin (1 mg/ml) (N8090, Solarbio, China) were added to the milk and fed for 5 days to eliminate as much as possible the gut microbial background of the piglets, followed by FMT or feeding of bacteria or metabolites. Feces were collected on day 8 from the uninfected group of Min pigs and Landrace pigs and treated as described previously. Briefly, fecal samples were placed in a sterile N2 incubator and mixed thoroughly. Then, cell filters were inserted to collect the bacteria and centrifuged at 600×g for 10 min. The samples were then added to 10% glycerol and stored at −80 °C [23 (link)]. Prior to FMT, PBS was used instead of glycerol, and the number of viable bacteria was subsequently counted by methylene blue staining. Two milliliters of the bacterial solution were mixed into the milk and fed to the piglets for three consecutive days, and the PEDV challenge occurred on day 0 by gavage.
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