Fmmyalf

Manufactured by GenScript

Sourced in United States

The FMMYALF is a laboratory equipment used for analytical purposes. It serves as a tool for various scientific investigations and procedures. The core function of this equipment is to provide accurate measurements and data analysis capabilities to support research and development activities in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Lsrfortessa flow cytometer, by FlowJo (1 mentions) Alexa fluor 488 anti human cd16, by BioLegend (1 mentions) Brilliant violet 421 anti human cd45, by BioLegend (1 mentions) Pe anti human cd62l, by BioLegend (1 mentions) Apc anti human cd63, by BioLegend (1 mentions)

2 protocols using fmmyalf

Neutrophil Activation and Surface Marker Analysis

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Isolated neutrophils were resuspended in PBS free from calcium and magnesium ions (PAA) at 10 × 10⁶/mL and stimulated for 2 hours with 100 nM fMLF (Sigma-AldrichK) or 100 nM fMMYALF (GenScript) or pretreated for 10 minutes with 2.5 µM FPR1 antagonist CsH (Enzo Scientific) before stimulation. Neutrophils were incubated for 1 hour at 4°C with antibodies to Brilliant Violet 421 anti-human CD45 (Clone: HI30), APC/Fire 750 anti-human CD11b (activated) (Clone: CBRM1/5), Alexa Fluor 488 anti-human CD16 (Clone: 3G8), PE anti-human CD62L (Clone: DREG-56), and APC anti-human CD63 (Clone: H5C6) (BioLegend). Samples were washed and resuspended in 2% FCS and incubated briefly with DAPI (1:1000) before analysis using a BD Bioscience LSR Fortessa flow cytometer and FlowJo software (version 10.1).

McAllister M.J., Hall R., Whelan R.J., Fischer L.J., Chuah C.S., Cartlidge P.D., Drury B., Rutherford D.G., Duffin R.M., Cartwright J.A., Dorward D.A., Rossi A.G, & Ho G.T. (2024). Formylated Peptide Receptor-1-Mediated Gut Inflammation as a Therapeutic Target in Inflammatory Bowel Disease. Crohn's & Colitis 360, 6(1), otae003.

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Neutrophil Oxidative Burst Assay

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Neutrophils, plated at 3 × 10⁵ cells/well, were incubated with a selective inhibitor of FPR1, cyclosporine H (CsH, 5 μM) for 30 min prior to the addition of stimuli, R848 (2.5 μg/ml), N-Formyl-Met-Leu-Phe (1 μM), formylated MT-ND6 peptide (1 μM) or plasma (1:100 dilution) for an additional 60 min. DHR 123 (0.5 μM), was added during last 30 min of incubation and ROS was analyzed by flow cytometry. Published sequence of formylated MT-ND6 peptide (f-MMYALF) [31 (link)] was synthesized commercially (GenScript USA, Inc.) and was tested as endotoxin-free.

Duvvuri B., Baddour A.A., Deane K.D., Feser M.L., Nelson J.L., Demoruelle M.K, & Lood C. (2021). Mitochondrial N-formyl methionine peptides associate with disease activity as well as contribute to neutrophil activation in patients with rheumatoid arthritis. Journal of autoimmunity, 119, 102630.

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