Reverse transcription polymerase chain reaction (RT-PCR) kits, Trizol, paraffin-embedded tissue RNA extraction kits (k1560-2), and reverse transcription cDNA synthesis kits (K1622) were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). High fidelity Taq enzyme, Taq DNA polymerase (RR066B), and fluorescence quantitative PCR kits (RR066B) were purchased from TaKaRa Bio, Inc. (Mountain View, CA, USA). HER2 (2242) and mouse anti-human CD44 monoclonal antibodies (14-0441) were purchased from Affymetrix, Inc (Santa Clara, CA, USA), while the quantitative real-time PCR instrument was purchased from ABI Corporation (Foster City, CA, USA).
Reverse transcription polymerase chain reaction rt pcr kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
Reverse transcription polymerase chain reaction (RT-PCR) kits are laboratory tools used to detect and amplify specific RNA sequences. The core function of these kits is to convert RNA into complementary DNA (cDNA) and then exponentially amplify the target cDNA sequence using the polymerase chain reaction (PCR) process.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (3 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Rna extraction kit, by Thermo Fisher Scientific (1 mentions)
Cell lysis buffer, by Beyotime (1 mentions)
Reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Cell lysis solution, by Beyotime (1 mentions)
Bicinchoninic acid bca protein quantification kit, by Beyotime (1 mentions)
Mgc 803, by National Collection of Authenticated Cell Cultures (1 mentions)
Primer synthesis, by Takara Bio (1 mentions)
4 protocols using reverse transcription polymerase chain reaction rt pcr kits
1
Quantitative Gene Expression Analysis
2
Resveratrol Inhibits Gastric Cancer
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Resveratrol, methylthiazolyl tetrazolium (MTT) assay (both from Sigma; Merck KGaA, Darmstadt, Germany); MGC-803 gastric cancer cell line (Chinese Academy of Sciences Cell Bank, Shanghai, China); rabbit anti-human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-catenin, c-myc, cyclin D1 polyclonal antibodies, and goat anti-rabbit HRP-conjugated secondary antibody (cat. nos. 10494-1-AP, 51067-2-AP, 10828-1-AP, 60186-1-Ig, SA00001-2; Wuhan Sanying Biotechnology, Wuhan, China); Dulbecco's modified Eagle's medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); RNA extraction kits, reverse transcription kits, reverse transcription-polymerase chain reaction (RT-PCR) kits (all from Invitrogen; Thermo Fisher Scientific, Inc.); primer synthesis (Takara Biotechnology Co., Ltd., Dalian, China); bicinchoninic acid (BCA) protein excretion kits, cell lysis buffer (both from Beyotime Institute of Biotechnology, Nantong, China). The study was approved by the Ethics Committee of Changchun University of Traditional Chinese Medicine Affiliated Hospital (Changchun, China).
3
Investigating Gastric Cancer Cell Lines
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Human gastric cancer cell lines MGC-803 (Cell Bank of Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) (Sigma, St. Louis, MO, USA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), PLK1 and E-cadherin human primary antibodies and horseradish peroxidase (HRP)-labeled second antibodies (all from Wuhan Sanying Biotechnology, Wuhan, China), primer synthesis (Takara Biotechnology Co., Ltd., Dalian, China), Dulbecco's modified Eagle's medium (DMEM) (Gibco Life Technologies, Carlsbad, CA, USA), ribonucleic acid (RNA) extraction kits, reverse transcription kits and reverse transcription-polymerase chain reaction (RT-PCR) kits (all from from Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA), bicinchoninic acid (BCA) protein quantification kits and cell lysis solution (all from Beyotime Institute of Biotechnology, Nantong, China). The study was approved by the Ethics Committee of The Second Affiliated Hospital of Zhengzhou University (Zhengzhou, China) and written informed consents were signed by the patients and/or guardians.
4
Colon Cancer Cell Line SW480 Assay
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Aesculetin, bromodeoxyuridine (BrdU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St. Louis, MO, USA); human colon cancer cell line SW480 (Cell Bank, Chinese Academy of Sciences, Shanghai, China); BrdU kit, rabbit anti-human glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cat. no. 10494-1-AP), β-catenin (cat. no. 17565-1-AP), c-Myc (cat. no. 10828-1-AP) and cyclin D1 (cat. no. 60186-1-Ig) primary polyclonal antibodies and goat anti-rabbit horseradish peroxidase (HRP)-labeled secondary polyclonal antibody (cat. no. SA00001-2) (ProteinTech Group Inc., Wuhan, China); Dulbecco's modified Eagle's medium (DMEM; Gibco, Carlsbad, CA, USA); ribonucleic acid (RNA) extraction kits, reverse transcription kits, reverse transcription-polymerase chain reaction (RT-PCR) kits (Invitrogen, Carlsbad, CA, USA); primer synthesis (Takara, Dalian, China); and human immunization (Biotechnology Research Institute, Nantong, China), bicinchoninic acid (BCA) protein quantification kits and cell lysate (Beyotime Institute of Biotechnology, Nantong, China) were used in the present study.
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