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Anti hnf4a clone c11f12

Manufactured by Cell Signaling Technology
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Anti-HNF4a (clone C11F12) is a laboratory antibody product produced by Cell Signaling Technology. The antibody specifically recognizes the HNF4a protein, which is a transcription factor involved in the regulation of gene expression. This antibody can be used for various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the HNF4a protein in biological samples.

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Lab products found in correlation

Ab92513, by R&D Systems (2 mentions) Ab34710, by Abcam (2 mentions) Anti cd10 human clone hi10a, by BioLegend (2 mentions) Anti mouse pdgfra af1062, by R&D Systems (2 mentions) Anti pan cytokeratin typei 2, by Thermo Fisher Scientific (2 mentions) Hpa012672, by Abcam (2 mentions) Mab350, by R&D Systems (2 mentions) Af647 donkey anti rabbit, by Jackson ImmunoResearch (2 mentions) Af488 donkey anti goat, by Jackson ImmunoResearch (2 mentions) Prolong gold, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

HNF4A (7 citations) Anti-HNF4a (4 citations)

2 protocols using anti hnf4a clone c11f12

1

Comprehensive Immunofluorescence Staining of Kidney Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney tissues were fixed in 4% formalin for 2 hours at RT and frozen in OCT after dehydration in 30% sucrose overnight. Using 5-10 μm cryosections, slides were blocked in 5% donkey serum followed by 1-hour incubation of the primary antibody, washing 3 times for 5 minutes in PBS and subsequent incubation of the secondary antibodies for 45 minutes. Following DAPI (4′,6′-′diamidino-2-phenylindole) staining (Roche, 1:10.000) the slides were mounted with ProLong Gold (Invitrogen, #P10144). The following antibodies were used: anti-mouse PDGFRa (AF1062, 1:100, R&D), anti-CD10 human (clone HI10a, 1:100, biolegend), anti-HNF4a (clone C11F12, 1:100, Cell Signalling), anti-Pan-Cytokeratin TypeI/II (Invitrogen, Ref. MA1-82041), anti-Dach1 (Sigma, HPA012672, 1:100), anti-Col1a1 (Abcam, ab34710, 1:100), anti-ERG (abcam, ab92513, 1:100), anti-CXCL12/SDF-1 (R&D, MAB350, 1:100), AF488 donkey anti goat (1:200, Jackson Immuno Research), AF647 donkey anti-rabbit (1:200, Jackson Immuno Research)
Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.
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2

Comprehensive Immunofluorescence Staining of Kidney Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney tissues were fixed in 4% formalin for 2 hours at RT and frozen in OCT after dehydration in 30% sucrose overnight. Using 5-10 μm cryosections, slides were blocked in 5% donkey serum followed by 1-hour incubation of the primary antibody, washing 3 times for 5 minutes in PBS and subsequent incubation of the secondary antibodies for 45 minutes. Following DAPI (4′,6′-′diamidino-2-phenylindole) staining (Roche, 1:10.000) the slides were mounted with ProLong Gold (Invitrogen, #P10144). The following antibodies were used: anti-mouse PDGFRa (AF1062, 1:100, R&D), anti-CD10 human (clone HI10a, 1:100, biolegend), anti-HNF4a (clone C11F12, 1:100, Cell Signalling), anti-Pan-Cytokeratin TypeI/II (Invitrogen, Ref. MA1-82041), anti-Dach1 (Sigma, HPA012672, 1:100), anti-Col1a1 (Abcam, ab34710, 1:100), anti-ERG (abcam, ab92513, 1:100), anti-CXCL12/SDF-1 (R&D, MAB350, 1:100), AF488 donkey anti goat (1:200, Jackson Immuno Research), AF647 donkey anti-rabbit (1:200, Jackson Immuno Research)
Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.
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